UniProt Accession

 GNAI1_MOUSE; B2RSH2 

Genbank Protein ID

 BC138862; BC138865 

Genbank Nucleotide ID

 AAI38863.1; AAI38866.1 

Protein Name

 Guanine nucleotide-binding protein G(i) subunit alpha-1 

Protein Synonyms/Alias

 Adenylate cyclase-inhibiting G alpha protein 

Gene Name

 Gnai1 

Gene Synonyms/Alias

 Gnai-1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
29	NLREDGEKAAREVKL	ubiquitination	[1]
35	EKAAREVKLLLLGAG	acetylation	[2]
35	EKAAREVKLLLLGAG	ubiquitination	[1]
54	STIVKQMKIIHEAGY	ubiquitination	[1]
67	GYSEEECKQYKAVVY	ubiquitination	[1]
92	IRAMGRLKIDFGDSA	ubiquitination	[1]
132	GVIKRLWKDSGVQAC	ubiquitination	[1]
180	DVLRTRVKTTGIVET	ubiquitination	[1]
192	VETHFTFKDLHFKMF	ubiquitination	[1]
197	TFKDLHFKMFDVGGQ	ubiquitination	[1]
248	NRMHESMKLFDSICN	ubiquitination	[1]
271	IILFLNKKDLFEEKI	ubiquitination	[1]
312	CQFEDLNKRKDTKEI	ubiquitination	[1]
349	VIIKNNLKDCGLF**	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (By similarity). 

Sequence Annotation

 NP_BIND 40 47 GTP (By similarity).
 NP_BIND 43 48 GTP (By similarity).
 NP_BIND 175 181 GTP (By similarity).
 NP_BIND 175 178 GTP (By similarity).
 NP_BIND 200 204 GTP (By similarity).
 NP_BIND 269 272 GTP (By similarity).
 METAL 47 47 Magnesium (By similarity).
 METAL 181 181 Magnesium (By similarity).
 BINDING 326 326 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine (By similarity).  

Keyword

 Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Palmitate; Reference proteome; Transducer. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 354 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0031821; F:G-protein coupled serotonin receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; IBA:RefGenome.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; ISS:UniProtKB. 

Interpro

 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00503; G-alpha 

SMART

 SM00275; G_alpha 

PROSITE

  

PRINTS

 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.