UniProt Accession

 MTRA_MYCTU; P0A5Z4; L0TES0; Q50447 

Genbank Protein ID

 U01971; AE000516; AL123456 

Genbank Nucleotide ID

 AAB07804.1; AAK47686.1; CCP46065.1 

Protein Name

 DNA-binding response regulator MtrA 

Protein Synonyms/Alias

  

Gene Name

 mtrA 

Gene Synonyms/Alias

 Rv3246c; MT3344; MTCY20B11.21c 

Created Date

 July 27, 2013 

Organism

 Mycobacterium tuberculosis 

NCBI Taxa ID

 1773 

Lysine Modification

Position	Peptide	Type	References
204	HVQRLRAKVEKDPEN	pupylation	[1]

Reference

 [1] Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .
 Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, Gygi SP, Darwin KH.
 PLoS One. 2010 Jan 6;5(1):e8589. [PMID: 20066036] 

Functional Description

 Member of the two-component regulatory system MtrA/MtrB. Binds direct repeat motifs of sequence 5'-GTCACAGCG-3', phosphorylation confers higher affinity. Overexpression decreases bacteria viability upon infection of human THP-1 macrophage cell line, due at least in part to impaired blockage of phagosome- lysosome fusion (upon infection bacteria usually remain in phagosomes). Infecting C57BL/6 mice with an overexpressing strain leads to an attentuated infection in both spleen and lungs. The level of dnaA mRNA increases dramatically. Binds the promoter of dnaA, fbpD, ripA and itself, as well as oriC, which it may regulate. Upon co-overexpression of MrtA and MtrB growth in macrophages is partially restored, dnaA expression is not induced, although mouse infections are still attenuated, suggesting that bacterial growth in macrophages requires an optimal ratio of MtrB to MtrA. 

Sequence Annotation

 DOMAIN 7 120 Response regulatory.
 METAL 13 13 Divalent cation.
 METAL 56 56 Divalent cation.
 METAL 59 59 Divalent cation; via carbonyl oxygen.
 MOD_RES 56 56 4-aspartylphosphate (Probable).
 CROSSLNK 207 207 Isoglutamyl lysine isopeptide (Lys-Gln)  

Keyword

 3D-structure; Complete proteome; Cytoplasm; DNA-binding; Isopeptide bond; Metal-binding; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Two-component regulatory system; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 228 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:MTBBASE.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
 GO:0044117; P:growth of symbiont in host; IMP:MTBBASE.
 GO:0035556; P:intracellular signal transduction; IEA:GOC.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:MTBBASE.
 GO:0006468; P:protein phosphorylation; IDA:MTBBASE.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011006; CheY-like_superfamily.
 IPR001867; Sig_transdc_resp-reg_C.
 IPR001789; Sig_transdc_resp-reg_receiver.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00072; Response_reg
 PF00486; Trans_reg_C 

SMART

 SM00448; REC
 SM00862; Trans_reg_C 

PROSITE

 PS50110; RESPONSE_REGULATORY 

PRINTS