UniProt Accession

 UBE2K_MOUSE; P61087; O54806; P27924; Q16721; Q9CVV9 

Genbank Protein ID

 AB011081; BC002013; BC085311; AK006316 

Genbank Nucleotide ID

 BAA24927.1; AAH02013.1; AAH85311.1; BAB24523.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 K 

Protein Synonyms/Alias

 Huntingtin-interacting protein 2; HIP-2; Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K; Ubiquitin-protein ligase 

Gene Name

 Ube2k 

Gene Synonyms/Alias

 Hip2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
24	LKSEETSKNQIKVDL	ubiquitination	[1]
61	GRYQLEIKIPETYPF	ubiquitination	[1]
72	TYPFNPPKVRFITKI	ubiquitination	[1]
142	KQNPEMFKQTARLWA	acetylation	[2]
164	VSSPEYTKKIENLCA	ubiquitination	[1]
165	SSPEYTKKIENLCAM	acetylation	[2, 3]
165	SSPEYTKKIENLCAM	succinylation	[3]
165	SSPEYTKKIENLCAM	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. Involved in stabilization of CASP12 during ER stress-mediated beta-amyloid neurotoxicity probably by inhibiting proteasome activity; in vitro ubiquitinates CASP12. 

Sequence Annotation

 DOMAIN 160 200 UBA.
 ACT_SITE 92 92 Glycyl thioester intermediate (By
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 14 14 N6-acetyllysine; alternate (By
 CROSSLNK 14 14 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 200 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0031625; F:ubiquitin protein ligase binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IEA:Compara.
 GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
 GO:0032434; P:regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:MGI. 

Interpro

 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00627; UBA
 PF00179; UQ_con 

SMART

 SM00165; UBA 

PROSITE

 PS50030; UBA
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS