CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023845
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rap guanine nucleotide exchange factor 2 
Protein Synonyms/Alias
 Neural RAP guanine nucleotide exchange protein; nRap GEP; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ-GEF1; RA-GEF 
Gene Name
 RAPGEF2 
Gene Synonyms/Alias
 KIAA0313; NRAPGEP; PDZGEF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
382IACAAKAKRRLMTLTubiquitination[1]
548RISILPQKPYNDIGIubiquitination[2]
688LSGRYYLKNNMETETubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP. 
Sequence Annotation
 DOMAIN 267 380 N-terminal Ras-GEF.
 DOMAIN 385 470 PDZ.
 DOMAIN 606 692 Ras-associating.
 DOMAIN 717 944 Ras-GEF.
 NP_BIND 135 254 cNMP.
 MOD_RES 418 418 Phosphoserine (By similarity).
 MOD_RES 420 420 Phosphoserine (By similarity).
 MOD_RES 644 644 Phosphothreonine; by PLK2 (By
 MOD_RES 806 806 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 933 933 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 1022 1022 Phosphoserine.
 MOD_RES 1176 1176 Phosphoserine; by PLK2 (By similarity).  
Keyword
 Cell membrane; Complete proteome; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1499 AA 
Protein Sequence
MKPLAIPANH GVMGQQEKHS LPADFTKLHL TDSLHPQVTH VSSSHSGCSI TSDSGSSSLS 60
DIYQATESEA GDMDLSGLPE TAVDSEDDDD EEDIERASDP LMSRDIVRDC LEKDPIDRTD 120
DDIEQLLEFM HQLPAFANMT MSVRRELCAV MVFAVVERAG TIVLNDGEEL DSWSVILNGS 180
VEVTYPDGKA EILCMGNSFG VSPTMDKEYM KGVMRTKVDD CQFVCIAQQD YCRILNQVEK 240
NMQKVEEEGE IVMVKEHREL DRTGTRKGHI VIKGTSERLT MHLVEEHSVV DPTFIEDFLL 300
TYRTFLSSPM EVGKKLLEWF NDPSLRDKVT RVVLLWVNNH FNDFEGDPAM TRFLEEFENN 360
LEREKMGGHL RLLNIACAAK AKRRLMTLTK PSREAPLPFI LLGGSEKGFG IFVDSVDSGS 420
KATEAGLKRG DQILEVNGQN FENIQLSKAM EILRNNTHLS ITVKTNLFVF KELLTRLSEE 480
KRNGAPHLPK IGDIKKASRY SIPDLAVDVE QVIGLEKVNK KSKANTVGGR NKLKKILDKT 540
RISILPQKPY NDIGIGQSQD DSIVGLRQTK HIPTALPVSG TLSSSNPDLL QSHHRILDFS 600
ATPDLPDQVL RVFKADQQSR YIMISKDTTA KEVVIQAIRE FAVTATPDQY SLCEVSVTPE 660
GVIKQRRLPD QLSKLADRIQ LSGRYYLKNN METETLCSDE DAQELLRESQ ISLLQLSTVE 720
VATQLSMRNF ELFRNIEPTE YIDDLFKLRS KTSCANLKRF EEVINQETFW VASEILRETN 780
QLKRMKIIKH FIKIALHCRE CKNFNSMFAI ISGLNLAPVA RLRTTWEKLP NKYEKLFQDL 840
QDLFDPSRNM AKYRNVLNSQ NLQPPIIPLF PVIKKDLTFL HEGNDSKVDG LVNFEKLRMI 900
AKEIRHVGRM ASVNMDPALM FRTRKKKWRS LGSLSQGSTN ATVLDVAQTG GHKKRVRRSS 960
FLNAKKLYED AQMARKVKQY LSNLELEMDE ESLQTLSLQC EPATNTLPKN PGDKKPVKSE 1020
TSPVAPRAGS QQKAQSLPQP QQQPPPAHKI NQGLQVPAVS LYPSRKKVPV KDLPPFGINS 1080
PQALKKILSL SEEGSLERHK KQAEDTISNA SSQLSSPPTS PQSSPRKGYT LAPSGTVDNF 1140
SDSGHSEISS RSSIVSNSSF DSVPVSLHDE RRQRHSVSIV ETNLGMGRME RRTMIEPDQY 1200
SLGSYAPMSE GRGLYATATV ISSPSTEELS QDQGDRASLD AADSGRGSWT SCSSGSHDNI 1260
QTIQHQRSWE TLPFGHTHFD YSGDPAGLWA SSSHMDQIMF SDHSTKYNRQ NQSRESLEQA 1320
QSRASWASST GYWGEDSEGD TGTIKRRGGK DVSIEAESSS LTSVTTEETK PVPMPAHIAV 1380
ASSTTKGLIA RKEGRYREPP PTPPGYIGIP ITDFPEGHSH PARKPPDYNV ALQRSRMVAR 1440
SSDTAGPSSV QQPHGHPTSS RPVNKPQWHK PNESDPRLAP YQSQGFSTEE DEDEQVSAV 1499 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; NAS:UniProtKB.
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0019992; F:diacylglycerol binding; NAS:UniProtKB.
 GO:0046582; F:Rap GTPase activator activity; IDA:UniProtKB.
 GO:0017034; F:Rap guanyl-nucleotide exchange factor activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:UniProtKB.
 GO:0019933; P:cAMP-mediated signaling; NAS:UniProtKB.
 GO:0000165; P:MAPK cascade; NAS:UniProtKB.
 GO:0048167; P:regulation of synaptic plasticity; ISS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:UniProtKB. 
Interpro
 IPR018490; cNMP-bd-like.
 IPR000595; cNMP-bd_dom.
 IPR001478; PDZ.
 IPR000159; Ras-assoc.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF00595; PDZ
 PF00788; RA
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00100; cNMP
 SM00228; PDZ
 SM00314; RA
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3
 PS50106; PDZ
 PS50200; RA
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER 
PRINTS