CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001065
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome subunit alpha type-3 
Protein Synonyms/Alias
 Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8; Proteasome subunit K 
Gene Name
 Psma3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
52GVVFGVEKLVLSKLYacetylation[1]
57VEKLVLSKLYEEGSNacetylation[1, 2, 3]
57VEKLVLSKLYEEGSNubiquitination[4]
110FGYNIPLKHLADRVAubiquitination[4]
179AAKTEIEKLQMKEMTubiquitination[4]
183EIEKLQMKEMTCRDVubiquitination[4]
206YIVHDEVKDKAFELEacetylation[5]
206YIVHDEVKDKAFELEubiquitination[4]
230GRHEIVPKDIREEAEubiquitination[4]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 57 57 N6-acetyllysine (By similarity).
 MOD_RES 206 206 N6-acetyllysine (By similarity).
 MOD_RES 230 230 N6-acetyllysine (By similarity).
 MOD_RES 243 243 Phosphoserine.
 MOD_RES 250 250 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleus; Phosphoprotein; Protease; Proteasome; Reference proteome; Threonine protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 255 AA 
Protein Sequence
MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE 60
EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV 120
HAYTLYSAVR PFGCSFMLGS YSANDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL 180
QMKEMTCRDV VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TKGRHEIVPK DIREEAEKYA 240
KESLKEEDES DDDNM 255 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005839; C:proteasome core complex; IDA:UniProtKB.
 GO:0019773; C:proteasome core complex, alpha-subunit complex; IEA:InterPro.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR000426; Proteasome_asu_N.
 IPR023332; Proteasome_suA-type.
 IPR001353; Proteasome_sua/b. 
Pfam
 PF00227; Proteasome
 PF10584; Proteasome_A_N 
SMART
 SM00948; Proteasome_A_N 
PROSITE
 PS00388; PROTEASOME_A_1
 PS51475; PROTEASOME_A_2 
PRINTS