CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009544
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 40S ribosomal protein S6 
Protein Synonyms/Alias
 Phosphoprotein NP33 
Gene Name
 RPS6 
Gene Synonyms/Alias
 OK/SW-cl.2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14FPATGCQKLIEVDDEubiquitination[1, 2]
30KLRTFYEKRMATEVAacetylation[3]
30KLRTFYEKRMATEVAubiquitination[2]
46DALGEEWKGYVVRISubiquitination[2, 4]
58RISGGNDKQGFPMKQubiquitination[1, 2, 4]
64DKQGFPMKQGVLTHGubiquitination[1, 2]
79RVRLLLSKGHSCYRPubiquitination[2]
116LNLVIVKKGEKDIPGubiquitination[1, 2, 5]
119VIVKKGEKDIPGLTDubiquitination[2]
143KRASRIRKLFNLSKEubiquitination[1]
149RKLFNLSKEDDVRQYubiquitination[1, 2, 4]
188TPRVLQHKRRRIALKubiquitination[4]
203KQRTKKNKEEAAEYAacetylation[3]
203KQRTKKNKEEAAEYAubiquitination[2, 4]
211EEAAEYAKLLAKRMKacetylation[3]
211EEAAEYAKLLAKRMKubiquitination[1, 2, 4, 5]
243SLRASTSKSESSQK*sumoylation[6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Targeted identification of SUMOylation sites in human proteins using affinity enrichment and paralog-specific reporter ions.
 Lamoliatte F, Bonneil E, Durette C, Caron-Lizotte O, Wildemann D, Zerweck J, Wenschuh H, Thibault P.
 Mol Cell Proteomics. 2013 Jun 7;. [PMID: 23750026] 
Functional Description
 May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. 
Sequence Annotation
 MOD_RES 211 211 N6-acetyllysine.
 MOD_RES 235 235 Phosphoserine; by RPS6KA1, RPS6KA3, DAPK1
 MOD_RES 236 236 Phosphoserine; by RPS6KA1, RPS6KA3, DAPK1
 MOD_RES 240 240 Phosphoserine.
 MOD_RES 242 242 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 246 246 Phosphoserine (By similarity).
 MOD_RES 247 247 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosomal protein. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 249 AA 
Protein Sequence
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG 60
FPMKQGVLTH GRVRLLLSKG HSCYRPRRTG ERKRKSVRGC IVDANLSVLN LVIVKKGEKD 120
IPGLTDTTVP RRLGPKRASR IRKLFNLSKE DDVRQYVVRK PLNKEGKKPR TKAPKIQRLV 180
TPRVLQHKRR RIALKKQRTK KNKEEAAEYA KLLAKRMKEA KEKRQEQIAK RRRLSSLRAS 240
TSKSESSQK 249 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0042593; P:glucose homeostasis; ISS:UniProtKB.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
 GO:0042274; P:ribosomal small subunit biogenesis; IMP:UniProtKB.
 GO:0006364; P:rRNA processing; IMP:UniProtKB.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0031929; P:TOR signaling cascade; IDA:UniProtKB.
 GO:0006414; P:translational elongation; TAS:Reactome.
 GO:0006413; P:translational initiation; TAS:Reactome.
 GO:0006415; P:translational termination; TAS:Reactome.
 GO:0019083; P:viral transcription; TAS:Reactome. 
Interpro
 IPR014401; Ribosomal_S6_euk.
 IPR001377; Ribosomal_S6e.
 IPR018282; Ribosomal_S6e_CS. 
Pfam
 PF01092; Ribosomal_S6e 
SMART
  
PROSITE
 PS00578; RIBOSOMAL_S6E 
PRINTS