CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003513
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-acetylglucosamine-6-phosphate deacetylase 
Protein Synonyms/Alias
 GlcNAc 6-P deacetylase 
Gene Name
 nagA 
Gene Synonyms/Alias
 b0677; JW0663 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
108TTSDELMKQGVRVMRacetylation[1]
120VMREYLAKHPNQALGacetylation[1]
139GPWLNLVKKGTHNPNacetylation[1]
347ARAIGVEKRLGTLAAacetylation[1]
368TAFTPDFKITKTIVNacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the deacetylation of N-acetyl-D-glucosamine-6- phosphate. In vitro, can also hydrolyze substrate analogs such as N-thioacetyl-D-glucosamine-6-phosphate, N-trifluoroacetyl-D- glucosamine-6-phosphate, N-acetyl-D-glucosamine-6-sulfate, N- acetyl-D-galactosamine-6-phosphate, and N-formyl-D-glucosamine-6- phosphate. 
Sequence Annotation
 REGION 219 220 Substrate binding.
 REGION 306 308 Substrate binding.
 ACT_SITE 273 273 Proton donor/acceptor.
 METAL 131 131 Zinc.
 METAL 195 195 Zinc; via tele nitrogen.
 METAL 216 216 Zinc; via tele nitrogen.
 BINDING 142 142 Substrate; via amide nitrogen.
 BINDING 227 227 Substrate.
 BINDING 251 251 Substrate; via tele nitrogen.  
Keyword
 3D-structure; Carbohydrate metabolism; Complete proteome; Hydrolase; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 382 AA 
Protein Sequence
MYALTQGRIF TGHEFLDDHA VVIADGLIKS VCPVAELPPE IEQRSLNGAI LSPGFIDVQL 60
NGCGGVQFND TAEAVSVETL EIMQKANEKS GCTNYLPTLI TTSDELMKQG VRVMREYLAK 120
HPNQALGLHL EGPWLNLVKK GTHNPNFVRK PDAALVDFLC ENADVITKVT LAPEMVPAEV 180
ISKLANAGIV VSAGHSNATL KEAKAGFRAG ITFATHLYNA MPYITGREPG LAGAILDEAD 240
IYCGIIADGL HVDYANIRNA KRLKGDKLCL VTDATAPAGA NIEQFIFAGK TIYYRNGLCV 300
DENGTLSGSS LTMIEGVRNL VEHCGIALDE VLRMATLYPA RAIGVEKRLG TLAAGKVANL 360
TAFTPDFKIT KTIVNGNEVV TQ 382 
Gene Ontology
 GO:0008448; F:N-acetylglucosamine-6-phosphate deacetylase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0006046; P:N-acetylglucosamine catabolic process; IDA:UniProtKB.
 GO:0019262; P:N-acetylneuraminate catabolic process; IEA:UniProtKB-UniPathway.
 GO:0051289; P:protein homotetramerization; IDA:UniProtKB. 
Interpro
 IPR006680; Amidohydro_1.
 IPR003764; GlcNAc_6-P_deAcase.
 IPR011059; Metal-dep_hydrolase_composite. 
Pfam
 PF01979; Amidohydro_1 
SMART
  
PROSITE
  
PRINTS