UniProt Accession

 MACD1_MOUSE; Q922B1 

Genbank Protein ID

 AK052998; BC008653 

Genbank Nucleotide ID

 BAC35234.1; AAH08653.1 

Protein Name

 O-acetyl-ADP-ribose deacetylase MACROD1 

Protein Synonyms/Alias

 MACRO domain-containing protein 1; Protein LRP16; [Protein ADP-ribosylglutamate] hydrolase 

Gene Name

 Macrod1 

Gene Synonyms/Alias

 Lrp16 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
94	LSTSTDWKEAKSFLK	acetylation	[1, 2, 3]
94	LSTSTDWKEAKSFLK	succinylation	[3]
94	LSTSTDWKEAKSFLK	ubiquitination	[4]
97	STDWKEAKSFLKGLS	acetylation	[1]
101	KEAKSFLKGLSDKQR	acetylation	[1, 2, 3]
101	KEAKSFLKGLSDKQR	succinylation	[3]
119	YFCKDFIKLKKIPTW	acetylation	[1]
127	LKKIPTWKETAKGLA	acetylation	[1, 2, 3]
127	LKKIPTWKETAKGLA	succinylation	[3]
136	TAKGLAVKVEDPKYK	acetylation	[2]
141	AVKVEDPKYKKDKQL	acetylation	[1]
146	DPKYKKDKQLNEKIS	acetylation	[1, 5]
151	KDKQLNEKISLYRGD	acetylation	[1]
161	LYRGDITKLEVDAIV	acetylation	[2, 6, 7]
207	LQNCETGKAKITCGY	acetylation	[3]
207	LQNCETGKAKITCGY	succinylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [7] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758] 

Functional Description

 Removes ADP-ribose from glutamate residues in proteins bearing a single ADP-ribose moiety. Inactive towards proteins bearing poly-ADP-ribose. Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen (By similarity). 

Sequence Annotation

 DOMAIN 139 320 Macro.
 REGION 157 159 Substrate binding (By similarity).
 REGION 170 172 Substrate binding (By similarity).
 REGION 177 182 Substrate binding (By similarity).
 REGION 265 271 Substrate binding (By similarity).
 BINDING 304 304 Substrate (By similarity).  

Keyword

 Complete proteome; DNA damage; Hydrolase; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 323 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0019213; F:deacetylase activity; IEA:Compara.
 GO:0016798; F:hydrolase activity, acting on glycosyl bonds; ISS:UniProtKB.
 GO:0051725; P:protein de-ADP-ribosylation; ISS:UniProtKB.
 GO:0042278; P:purine nucleoside metabolic process; IEA:Compara.
 GO:0006974; P:response to DNA damage stimulus; ISS:UniProtKB. 

Interpro

 IPR002589; A1pp. 

Pfam

 PF01661; Macro 

SMART

 SM00506; A1pp 

PROSITE

 PS51154; MACRO 

PRINTS