CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017009
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitofusin-1 
Protein Synonyms/Alias
 Fzo homolog; Transmembrane GTPase MFN1 
Gene Name
 MFN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AEPVSPLKHFVLAKKubiquitination[1, 2]
61LVEMQGYKDKLSIIGubiquitination[3]
63EMQGYKDKLSIIGEVubiquitination[1, 3]
133MTEGSDEKKSVKTVNubiquitination[3]
150AHALHMDKDLKAGCLubiquitination[1, 2, 3]
153LHMDKDLKAGCLVRVubiquitination[3]
222TEKHFFHKVNERLSKubiquitination[2]
229KVNERLSKPNIFILNubiquitination[1, 2]
286RIFFVSAKEVLSARKubiquitination[2]
336SQSAVKTKFEQHTIRubiquitination[2]
345EQHTIRAKQILATVKubiquitination[2]
365VNLAAEDKRHYSVEEubiquitination[2, 3]
395NLLTLDVKKKIKEVTubiquitination[1, 2, 3, 4]
396LLTLDVKKKIKEVTEubiquitination[2]
397LTLDVKKKIKEVTEEubiquitination[2]
439HPNPDVLKIYKSELNubiquitination[2, 3]
442PDVLKIYKSELNKHIubiquitination[5]
447IYKSELNKHIEDGMGubiquitination[2, 3, 5]
500HTLIPCKKFDLSYNLubiquitination[1]
653FVNYATEKLRMIVSSubiquitination[2, 3]
689QQVDITQKQLEEEIAubiquitination[2]
707KEIDQLEKIQNNSKLubiquitination[2]
713EKIQNNSKLLRNKAVubiquitination[2]
718NSKLLRNKAVQLENEubiquitination[2, 3]
731NELENFTKQFLPSSNubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. 
Sequence Annotation
 NP_BIND 82 89 GTP (Probable).
 NP_BIND 178 182 GTP (Probable).
 NP_BIND 237 240 GTP (Probable).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; GTP-binding; Hydrolase; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 741 AA 
Protein Sequence
MAEPVSPLKH FVLAKKAITA IFDQLLEFVT EGSHFVEATY KNPELDRIAT EDDLVEMQGY 60
KDKLSIIGEV LSRRHMKVAF FGRTSSGKSS VINAMLWDKV LPSGIGHITN CFLSVEGTDG 120
DKAYLMTEGS DEKKSVKTVN QLAHALHMDK DLKAGCLVRV FWPKAKCALL RDDLVLVDSP 180
GTDVTTELDS WIDKFCLDAD VFVLVANSES TLMNTEKHFF HKVNERLSKP NIFILNNRWD 240
ASASEPEYME DVRRQHMERC LHFLVEELKV VNALEAQNRI FFVSAKEVLS ARKQKAQGMP 300
ESGVALAEGF HARLQEFQNF EQIFEECISQ SAVKTKFEQH TIRAKQILAT VKNIMDSVNL 360
AAEDKRHYSV EEREDQIDRL DFIRNQMNLL TLDVKKKIKE VTEEVANKVS CAMTDEICRL 420
SVLVDEFCSE FHPNPDVLKI YKSELNKHIE DGMGRNLADR CTDEVNALVL QTQQEIIENL 480
KPLLPAGIQD KLHTLIPCKK FDLSYNLNYH KLCSDFQEDI VFPFSLGWSS LVHRFLGPRN 540
AQRVLLGLSE PIFQLPRSLA STPTAPTTPA TPDNASQEEL MITLVTGLAS VTSRTSMGII 600
IVGGVIWKTI GWKLLSVSLT MYGALYLYER LSWTTHAKER AFKQQFVNYA TEKLRMIVSS 660
TSANCSHQVK QQIATTFARL CQQVDITQKQ LEEEIARLPK EIDQLEKIQN NSKLLRNKAV 720
QLENELENFT KQFLPSSNEE S 741 
Gene Ontology
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; NAS:UniProtKB.
 GO:0008053; P:mitochondrial fusion; IDA:BHF-UCL. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR006884; Fzo/mitofusin_HR2.
 IPR027088; Mitofusin-1.
 IPR027094; Mitofusin_fam.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N
 PF04799; Fzo_mitofusin 
SMART
  
PROSITE
  
PRINTS