UniProt Accession

 IFIT3_HUMAN; O14879; Q99634; Q9BSK7 

Genbank Protein ID

 AF026939; AF083470; U52513; BT007284; BC001383; BC004977 

Genbank Nucleotide ID

 AAB95160.1; AAC63524.1; AAB40606.1; AAP35948.1; AAH01383.1; AAH04977.1 

Protein Name

 Interferon-induced protein with tetratricopeptide repeats 3 

Protein Synonyms/Alias

 IFIT-3; CIG49; ISG-60; Interferon-induced 60 kDa protein; IFI-60K; Interferon-induced protein with tetratricopeptide repeats 4; IFIT-4; Retinoic acid-induced gene G protein; P60; RIG-G 

Gene Name

 IFIT3 

Gene Synonyms/Alias

 CIG-49; IFI60; IFIT4; ISG60 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MSEVTKNSLEKIL	ubiquitination	[1]
156	CGRNERAKVCFEKAL	ubiquitination	[1]
249	DVLRSAAKFYRRKGD	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS- mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin- dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2. 

Sequence Annotation

 REPEAT 51 84 TPR 1.
 REPEAT 94 127 TPR 2.
 REPEAT 136 169 TPR 3.
 REPEAT 172 206 TPR 4.
 REPEAT 207 240 TPR 5.
 REPEAT 241 274 TPR 6.
 REPEAT 415 448 TPR 7.
 REPEAT 450 481 TPR 8.
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 237 237 Phosphoserine.
 MOD_RES 478 478 Phosphoserine.  

Keyword

 Antiviral defense; Complete proteome; Cytoplasm; Immunity; Innate immunity; Mitochondrion; Phosphoprotein; Reference proteome; Repeat; TPR repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 490 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0035457; P:cellular response to interferon-alpha; IEA:InterPro.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0009615; P:response to virus; IMP:UniProtKB.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 

Interpro

 IPR024122; Interferon-induced_IFIT3.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF00515; TPR_1
 PF13181; TPR_8 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS