CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012550
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Angiopoietin-1 
Protein Synonyms/Alias
 ANG-1 
Gene Name
 ANGPT1 
Gene Synonyms/Alias
 KIAA0003 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
186QQTNEILKIHEKNSLacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. 
Sequence Annotation
 DOMAIN 277 497 Fibrinogen C-terminal.
 CARBOHYD 92 92 N-linked (GlcNAc...) (Potential).
 CARBOHYD 122 122 N-linked (GlcNAc...) (Potential).
 CARBOHYD 154 154 N-linked (GlcNAc...) (Potential).
 CARBOHYD 243 243 N-linked (GlcNAc...) (Potential).
 CARBOHYD 295 295 N-linked (GlcNAc...) (Potential).
 DISULFID 286 315 By similarity.
 DISULFID 439 452 By similarity.  
Keyword
 3D-structure; Alternative splicing; Angiogenesis; Coiled coil; Complete proteome; Developmental protein; Differentiation; Disulfide bond; Glycoprotein; Polymorphism; Reference proteome; Secreted; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 498 AA 
Protein Sequence
MTVFLSFAFL AAILTHIGCS NQRRSPENSG RRYNRIQHGQ CAYTFILPEH DGNCRESTTD 60
QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL ENYIVENMKS EMAQIQQNAV 120
QNHTATMLEI GTSLLSQTAE QTRKLTDVET QVLNQTSRLE IQLLENSLST YKLEKQLLQQ 180
TNEILKIHEK NSLLEHKILE MEGKHKEELD TLKEEKENLQ GLVTRQTYII QELEKQLNRA 240
TTNNSVLQKQ QLELMDTVHN LVNLCTKEGV LLKGGKREEE KPFRDCADVY QAGFNKSGIY 300
TIYINNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN PSGEYWLGNE 360
FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN YRLYLKGHTG TAGKQSSLIL 420
HGADFSTKDA DNDNCMCKCA LMLTGGWWFD ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK 480
GPSYSLRSTT MMIRPLDF 498 
Gene Ontology
 GO:0005615; C:extracellular space; IDA:UniProtKB.
 GO:0045121; C:membrane raft; IDA:UniProtKB.
 GO:0005902; C:microvillus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0007171; P:activation of transmembrane receptor protein tyrosine kinase activity; IDA:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0031589; P:cell-substrate adhesion; IEA:Compara.
 GO:0072012; P:glomerulus vasculature development; ISS:UniProtKB.
 GO:0030097; P:hemopoiesis; IEA:Compara.
 GO:0030210; P:heparin biosynthetic process; IDA:UniProtKB.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0007162; P:negative regulation of cell adhesion; IDA:UniProtKB.
 GO:2000352; P:negative regulation of endothelial cell apoptotic process; IDA:UniProtKB.
 GO:0043116; P:negative regulation of vascular permeability; IDA:UniProtKB.
 GO:0050918; P:positive chemotaxis; IDA:UniProtKB.
 GO:0043536; P:positive regulation of blood vessel endothelial cell migration; IDA:UniProtKB.
 GO:0045785; P:positive regulation of cell adhesion; IEA:Compara.
 GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:UniProtKB.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IEA:Compara.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:UniProtKB.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IDA:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0002092; P:positive regulation of receptor internalization; IDA:UniProtKB.
 GO:0034394; P:protein localization to cell surface; IDA:UniProtKB.
 GO:0014842; P:regulation of satellite cell proliferation; IDA:UniProtKB.
 GO:0002040; P:sprouting angiogenesis; IDA:UniProtKB.
 GO:0048014; P:Tie signaling pathway; IDA:UniProtKB. 
Interpro
 IPR014716; Fibrinogen_a/b/g_C_1.
 IPR014715; Fibrinogen_a/b/g_C_2.
 IPR002181; Fibrinogen_a/b/g_C_dom.
 IPR020837; Fibrinogen_CS. 
Pfam
 PF00147; Fibrinogen_C 
SMART
 SM00186; FBG 
PROSITE
 PS00514; FIBRINOGEN_C_1
 PS51406; FIBRINOGEN_C_2 
PRINTS