UniProt Accession

 IMA1_MOUSE; P52293; Q64292 

Genbank Protein ID

 D55720; U12270; U34229; BC003274; BC006720 

Genbank Nucleotide ID

 BAA09536.1; AAA85281.1; AAC52451.1; AAH03274.1; AAH06720.1 

Protein Name

 Importin subunit alpha-1 

Protein Synonyms/Alias

 Importin alpha P1; Karyopherin subunit alpha-2; Pendulin; Pore targeting complex 58 kDa subunit; PTAC58; RAG cohort protein 1; SRP1-alpha 

Gene Name

 Kpna2 

Gene Synonyms/Alias

 Rch1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
108	RKLLSREKQPPIDNI	ubiquitination	[1]
240	LSNLCRNKNPAPPLD	ubiquitination	[1]
348	PSLLTNPKTNIQKEA	ubiquitination	[1]
486	HENESVYKASLNLIE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 

Sequence Annotation

 DOMAIN 2 60 IBB.
 REPEAT 71 111 ARM 1; truncated.
 REPEAT 112 151 ARM 2.
 REPEAT 152 193 ARM 3.
 REPEAT 200 244 ARM 4.
 REPEAT 246 282 ARM 5.
 REPEAT 283 322 ARM 6.
 REPEAT 325 364 ARM 7.
 REPEAT 367 409 ARM 8.
 REPEAT 410 456 ARM 9.
 REPEAT 457 496 ARM 10; atypical.
 REGION 142 238 NLS binding site (major).
 REGION 315 403 NLS binding site (minor).
 MOTIF 45 54 Nuclear localization signal.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 62 62 Phosphoserine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 529 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006606; P:protein import into nucleus; IEA:InterPro. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR002652; Importin-a_IBB.
 IPR024931; Importing_su_alpha. 

Pfam

 PF00514; Arm
 PF01749; IBB 

SMART

 SM00185; ARM 

PROSITE

 PS50176; ARM_REPEAT
 PS51214; IBB 

PRINTS