UniProt Accession

 E7EQR6_HUMAN; E7EQR6 

Genbank Protein ID

 AL135914 

Genbank Nucleotide ID

  

Protein Name

 T-complex protein 1 subunit alpha 

Protein Synonyms/Alias

  

Gene Name

 TCP1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
44	VNSVNILKAHGRSQM	ubiquitination	[1, 2, 3, 4, 5, 6]
78	PKRIVNAKIACLDFS	ubiquitination	[4, 6]
88	CLDFSLQKTKMKLGV	ubiquitination	[3, 4]
90	DFSLQKTKMKLGVQV	ubiquitination	[4]
92	SLQKTKMKLGVQVVI	ubiquitination	[4]
104	VVITDPEKLDQIRQR	ubiquitination	[1, 4, 6, 7]
210	DDELILIKNTKARTS	acetylation	[6, 8]
210	DDELILIKNTKARTS	ubiquitination	[3, 4, 6]
213	LILIKNTKARTSASI	ubiquitination	[4, 6]
245	HDALCVVKRVLESKS	acetylation	[6, 8, 9, 10]
245	HDALCVVKRVLESKS	ubiquitination	[2, 3, 4, 6]
311	DSTDLVAKLRAFHNE	acetylation	[8]
311	DSTDLVAKLRAFHNE	ubiquitination	[1, 4, 5, 6]
329	NPERKNLKWIGLDLS	acetylation	[6]
329	NPERKNLKWIGLDLS	ubiquitination	[3, 4, 5, 6, 11]
339	GLDLSNGKPRDNKQA	ubiquitination	[1, 3, 4, 5, 6, 11]
344	NGKPRDNKQAGVFEP	ubiquitination	[1, 3, 4, 6]
355	VFEPTIVKVKSLKFA	ubiquitination	[4, 6]
357	EPTIVKVKSLKFATE	ubiquitination	[4]
360	IVKVKSLKFATEAAI	ubiquitination	[2, 3, 5]
377	LRIDDLIKLHPESKD	acetylation	[6, 8]
377	LRIDDLIKLHPESKD	ubiquitination	[1, 2, 4, 5, 6, 11]
383	IKLHPESKDDKHGSY	acetylation	[6]
383	IKLHPESKDDKHGSY	ubiquitination	[1, 2, 4, 5, 6, 11]
386	HPESKDDKHGSYEDA	acetylation	[6, 8]
386	HPESKDDKHGSYEDA	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 11, 12]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [10] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [11] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [12] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 401 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IDA:HPA.
 GO:0005832; C:chaperonin-containing T-complex; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005720; C:nuclear heterochromatin; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000242; C:pericentriolar material; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006457; P:protein folding; IEA:InterPro. 

Interpro

 IPR012715; Chap_CCT_alpha.
 IPR017998; Chaperone_TCP-1.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

  

PRINTS

 PR00304; TCOMPLEXTCP1.