CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006838
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Valine/tyrosine/tryptophan amino-acid permease 1 
Protein Synonyms/Alias
 Tyrosine and tryptophan amino acid transporter 1 
Gene Name
 TAT1 
Gene Synonyms/Alias
 TAP1; VAP1; YBR069C; YBR0710 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
39RDFTITEKQDEVSGQubiquitination[1, 2, 3, 4, 5]
56EPRRTDSKSILQRKCubiquitination[5]
87SELESQEKNNLTKSIubiquitination[5]
92QEKNNLTKSIKSRHLubiquitination[5]
591VDDNDDLKEYENSESubiquitination[5]
Reference
 [1] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [5] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 High-affinity transport of valine and tyrosine. Low- affinity transport of tryptophan. Can also transport L-cysteine. 
Sequence Annotation
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 84 84 Phosphoserine.
 CROSSLNK 39 39 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Amino-acid transport; Complete proteome; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 619 AA 
Protein Sequence
MDDSVSFIAK EASPAQYSHS LHERTHSEKQ KRDFTITEKQ DEVSGQTAEP RRTDSKSILQ 60
RKCKEFFDSF KRQLPPDRNS ELESQEKNNL TKSIKSRHLV MISLGTGIGT GLLVGNGQVL 120
GTAGPAGLVL GYGIASIMLY CIIQAAGELG LCYAGLTGNY TRYPSILVDP SLGFAVSVVY 180
TIQWLTVLPL QLVTAAMTVK YWTSVNADIF VAVVFVFVII INLFGSRGYA EAEFIFNSCK 240
ILMVIGFVIL AIIINCGGAG DRRYIGAEYW HNPGPFAHGF KGVCTVFCYA AFSYGGIEVL 300
LLSAAEQENP TKSIPNACKK VVYRILLIYM LTTILVCFLV PYNSDELLGS SDSSGSHASP 360
FVIAVASHGV KVVPHFINAV ILISVISVAN SSLYSGPRLL LSLAEQGVLP KCLAYVDRNG 420
RPLLCFFVSL VFGCIGFVAT SDAEEQVFTW LLAISSLSQL FIWMSMSLSH IRFRDAMAKQ 480
GRSMNEVGYK AQTGYWGSWL AVLIAIFFLV CQFWVAIAPV NEHGKLNVKV FFQNYLAMPI 540
VLFAYFGHKI YFKSWSFWIP AEKIDLDSHR NIFVSPSLTE IDKVDDNDDL KEYENSESSE 600
NPNSSRSRKF FKRMTNFWC 619 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; ISS:SGD.
 GO:0022893; F:low-affinity tryptophan transmembrane transporter activity; IGI:SGD.
 GO:0015828; P:tyrosine transport; IMP:SGD. 
Interpro
 IPR004841; AA-permease_dom.
 IPR002293; AA/rel_permease1.
 IPR004762; Amino_acid_permease_fungi.
 IPR004840; Amoino_acid_permease_CS. 
Pfam
 PF00324; AA_permease 
SMART
  
PROSITE
 PS00218; AMINO_ACID_PERMEASE_1 
PRINTS