CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016019
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein VPRBP 
Protein Synonyms/Alias
 DDB1- and CUL4-associated factor 1 
Gene Name
 Vprbp 
Gene Synonyms/Alias
 Dcaf1; Kiaa0800 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
700NRISSIGKFISGTPRacetylation[1]
818LIERVSGKPLLIGTDubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the Cul4a-Rbx1-Ddb1-Vprbp/Dcaf1 E3 ubiquitin-protein ligase complex, Vprbp/Dcaf1 may function as the substrate recognition module within this complex (By similarity). Associated with chromatin in a Ddb1-independent and cell cycle- dependent manner, Vprbp/Dcaf1 is recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis (By similarity). 
Sequence Annotation
 DOMAIN 845 877 LisH.
 REPEAT 1090 1129 WD 1.
 REPEAT 1132 1173 WD 2.
 REPEAT 1175 1212 WD 3.
 REPEAT 1214 1246 WD 4.
 REPEAT 1247 1289 WD 5.
 REGION 1090 1173 WD repeat-like region.
 REGION 1417 1506 Interaction with NF2 (By similarity).
 MOTIF 1241 1248 DWD box 1 (By similarity).
 MOTIF 1277 1284 DWD box 2 (By similarity).
 MOD_RES 887 887 Phosphothreonine (By similarity).
 MOD_RES 894 894 Phosphoserine (By similarity).
 MOD_RES 897 897 Phosphoserine (By similarity).
 MOD_RES 978 978 Phosphoserine (By similarity).
 MOD_RES 999 999 Phosphoserine (By similarity).
 CROSSLNK 279 279 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1506 AA 
Protein Sequence
MTTVVVHVDS KAELTTLLEQ WEKDHGSGQD MVPILTRMSE LIEKETEEYR KGDPDPFDDR 60
HPGRADPECM LGHLLRILFK NDDFMNALVN AYVMTSREPP LNTAACRLLL DIMPGLETAV 120
VFQEKEGIVE NLFKWAREAD QPLRTYSTGL LGGAMENQDI AANYRDENSQ LVAIVLRRLR 180
ELQLQEVALR QDSKRPSPRK LSSEPLLPLD EEAVDMDYGD MAVDVVDGEQ ESSRDMEISF 240
RLDSSHKTSS RVNSATKPEE GGLKKNKSAK HGDRENFRKA KQKLGFSSSD PDRVFVELSN 300
SSWSEMSPWV IGTNYTLYPM TPAIEQRLIL QYLTPLGEYQ ELLPIFMQLG SRELMMFYID 360
LKQTNDVLLT FEALKHLASL LLHNKFATEF VAHGGVQKLL EIPRPSMAAT GVSMCLYYLS 420
YNQDAMERVC MHPHNVLSDV VNYTLWLMEC SHASGCCHAT MFFSICFSFR AVLELFDRYD 480
GLRRLVNLIS TLEILNLEDQ GALLSDDEIF ASRQTGKHTC MALRKYFEAH LAIKLEQVKQ 540
SLQRTEGGIL VHPQPPYKAC SYTHEQIVEM MEFLIEYGPA QLYWEPAEVF LKLSCVQLLL 600
QLISIACNWK TYYARNDTVR FALDVLAILT VVPKIQLQLA ESVDVLDEAG SAVSTVGISI 660
ILGVAEGEFF IHDAEIQKSA LQIIINCVCG PDNRISSIGK FISGTPRRKL SQTPKSSEHT 720
LAKMWNVVQS NNGIKVLLSL LSIKMPITDA DQIRALACKA LVGLSRSSTV RQIISKLPLF 780
SSCQIQQLMK EPVLQDKRSD HVKFCKYAAE LIERVSGKPL LIGTDVSLAR LQKADVVAQS 840
RISFPEKELL LLIRNHLISK GLGETATVLT READLPMTAA SHSSAFTPVT AAASPVSLPR 900
TPRIANGIAS RLGSHATVGA SAPSAPPAHP PPRPPQGSLP LPGPSYAGNS PLIGRISFIR 960
ERPSPCNGRK IRVLRQKSDH GAYSQSPAIK KQLDRHLPSP PTLDSIITEY LREQHARCKN 1020
PVATCPPFSL FTPHQCPEPK QRRQAPINFT SRLNRRASFP KYGGVDGGCF DRHLIFSRFR 1080
PISVFREANE DESGFTCCAF SARERFLMLG TCTGQLKLYN VFSGQEEASY NCHNSAITHL 1140
EPSRDGSLLL TSATWSQPLS ALWGMKSVFD MKHSFTEDHY VEFSKHSQDR VIGTKGDIAH 1200
IYDIQTGNKL LTLFNPDLAN NYKRNCATFN PTDDLVLNDG VLWDVRSAQA IHKFDKFNMN 1260
ISGVFHPNGL EVIINTEIWD LRTFHLLHTV PALDQCRVVF NHTGTVMYGA MLQADDEDDL 1320
LEERMKSPFG SSFRTFNATD YKPIATIDVK RNIFDLCTDT KDCYLAVIEN QGSMDALNMD 1380
TVCRLYEVGR QRLAEDEDEE EDQEEEEQEE EDDDEDDDDT DDLDELDTDQ LLEAELEEDD 1440
NNENAGEDGD NDFSPSDEEL ANLLEEGEEG EDEDSDADEE VELILGDTDS SDNSDLEDDI 1500
ILSLNE 1506 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR006594; LisH_dimerisation.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom. 
Pfam
  
SMART
 SM00667; LisH 
PROSITE
 PS50896; LISH
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS