CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022891
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCR4-NOT transcription complex subunit 8 
Protein Synonyms/Alias
 CAF1-like protein; CALIFp; CAF2; CCR4-associated factor 8; Caf1b 
Gene Name
 CNOT8 
Gene Synonyms/Alias
 CALIF; POP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
200YDVKYLMKSCKNLKGubiquitination[1]
206MKSCKNLKGGLQEVAubiquitination[1, 2, 3, 4, 5, 6, 7]
242GMAFFRMKELFFEDSubiquitination[1, 3, 5, 6]
254EDSIDDAKYCGRLYGubiquitination[1]
269LGTGVAQKQNEDVDSubiquitination[1, 3, 4, 5, 6]
280DVDSAQEKMSILAIIubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity. 
Sequence Annotation
 METAL 40 40 Divalent metal cation 1; catalytic (By
 METAL 40 40 Divalent metal cation 2; catalytic (By
 METAL 42 42 Divalent metal cation 2; catalytic (By
 METAL 161 161 Divalent metal cation 1; catalytic (By
 METAL 230 230 Divalent metal cation 2; catalytic (By  
Keyword
 Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Metal-binding; Nuclease; Nucleus; Polymorphism; Reference proteome; Repressor; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 292 AA 
Protein Sequence
MPAALVENSQ VICEVWASNL EEEMRKIREI VLSYSYIAMD TEFPGVVVRP IGEFRSSIDY 60
QYQLLRCNVD LLKIIQLGLT FTNEKGEYPS GINTWQFNFK FNLTEDMYSQ DSIDLLANSG 120
LQFQKHEEEG IDTLHFAELL MTSGVVLCDN VKWLSFHSGY DFGYMVKLLT DSRLPEEEHE 180
FFHILNLFFP SIYDVKYLMK SCKNLKGGLQ EVADQLDLQR IGRQHQAGSD SLLTGMAFFR 240
MKELFFEDSI DDAKYCGRLY GLGTGVAQKQ NEDVDSAQEK MSILAIINNM QQ 292 
Gene Ontology
 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; IDA:UniProtKB.
 GO:0035195; P:gene silencing by miRNA; TAS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR027212; Cnot8.
 IPR006941; RNase_CAF1.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF04857; CAF1 
SMART
  
PROSITE
  
PRINTS