CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021732
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Omega-amidase NIT2 
Protein Synonyms/Alias
 Nitrilase homolog 2 
Gene Name
 Nit2 
Gene Synonyms/Alias
 D16Ertd502e 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
68IPGESTQKLSEVAKEacetylation[1, 2, 3, 4, 5, 6, 7, 8, 9]
68IPGESTQKLSEVAKEsuccinylation[8]
74QKLSEVAKESSIYLIacetylation[9]
74QKLSEVAKESSIYLIubiquitination[10]
109PDGSLLVKHRKIHLFacetylation[4]
112SLLVKHRKIHLFDIDubiquitination[10]
123FDIDVPGKITFQESKacetylation[8]
123FDIDVPGKITFQESKsuccinylation[8]
130KITFQESKTLSPGDSacetylation[8]
130KITFQESKTLSPGDSsuccinylation[8]
130KITFQESKTLSPGDSubiquitination[10]
249LYSDIDLKKLAEIRQacetylation[5, 9]
249LYSDIDLKKLAEIRQubiquitination[10]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [8] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [9] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [10] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha- ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. 
Sequence Annotation
 DOMAIN 4 270 CN hydrolase.
 ACT_SITE 43 43 Proton acceptor (Potential).
 ACT_SITE 112 112 Potential.
 ACT_SITE 153 153 Nucleophile (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 276 AA 
Protein Sequence
MSTFRLALIQ LQVSSIKSDN LTRACSLVRE AAKQGANIVS LPECFNSPYG TTYFPDYAEK 60
IPGESTQKLS EVAKESSIYL IGGSIPEEDA GKLYNTCSVF GPDGSLLVKH RKIHLFDIDV 120
PGKITFQESK TLSPGDSFST FDTPYCKVGL GICYDMRFAE LAQIYAQRGC QLLVYPGAFN 180
LTTGPAHWEL LQRARAVDNQ VYVATASPAR DDKASYVAWG HSTVVDPWGQ VLTKAGTEET 240
ILYSDIDLKK LAEIRQQIPI LKQKRADLYT VESKKP 276 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0050152; F:omega-amidase activity; IEA:EC.
 GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. 
Interpro
 IPR003010; C-N_Hydrolase. 
Pfam
 PF00795; CN_hydrolase 
SMART
  
PROSITE
 PS50263; CN_HYDROLASE
 PS01227; UPF0012 
PRINTS