CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023235
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase CHIP 
Protein Synonyms/Alias
 Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; STIP1 homology and U box-containing protein 1 
Gene Name
 STUB1 
Gene Synonyms/Alias
 CHIP; PP1131 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22AGGGSPEKSPSAQELubiquitination[1]
221LFSQVDEKRKKRDIPubiquitination[1]
255SGITYDRKDIEEHLQubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. 
Sequence Annotation
 REPEAT 26 59 TPR 1.
 REPEAT 60 93 TPR 2.
 REPEAT 95 127 TPR 3.
 DOMAIN 226 300 U-box.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 25 25 Phosphoserine (By similarity).
 MOD_RES 273 273 Phosphoserine.
 CROSSLNK 2 2 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 22 22 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 221 221 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 255 255 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Isopeptide bond; Ligase; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 303 AA 
Protein Sequence
MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA 60
VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA 120
YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC 180
QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM 240
REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV 300
EDY 303 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0042405; C:nuclear inclusion body; IDA:BHF-UCL.
 GO:0031371; C:ubiquitin conjugating enzyme complex; TAS:HGNC.
 GO:0000151; C:ubiquitin ligase complex; IDA:UniProtKB.
 GO:0030544; F:Hsp70 protein binding; IDA:HGNC.
 GO:0051879; F:Hsp90 protein binding; IDA:BHF-UCL.
 GO:0051787; F:misfolded protein binding; IDA:BHF-UCL.
 GO:0030674; F:protein binding, bridging; TAS:HGNC.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0046332; F:SMAD binding; IDA:HGNC.
 GO:0030911; F:TPR domain binding; IDA:HGNC.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; ISS:UniProtKB.
 GO:0071218; P:cellular response to misfolded protein; IDA:BHF-UCL.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006515; P:misfolded or incompletely synthesized protein catabolic process; IDA:BHF-UCL.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0090035; P:positive regulation of chaperone-mediated protein complex assembly; IDA:BHF-UCL.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:HGNC.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:BHF-UCL.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:HGNC.
 GO:0051865; P:protein autoubiquitination; IDA:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; IDA:UniProtKB.
 GO:0051604; P:protein maturation; TAS:HGNC.
 GO:0031943; P:regulation of glucocorticoid metabolic process; IDA:HGNC.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0030579; P:ubiquitin-dependent SMAD protein catabolic process; IDA:HGNC. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat.
 IPR003613; Ubox_domain.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00515; TPR_1
 PF04564; U-box 
SMART
 SM00028; TPR
 SM00504; Ubox 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS