UniProt Accession

 GNMT_RAT; P13255 

Genbank Protein ID

 X06150; X07833 

Genbank Nucleotide ID

 CAA29508.1; CAA30686.1 

Protein Name

 Glycine N-methyltransferase 

Protein Synonyms/Alias

 Folate-binding protein 

Gene Name

 Gnmt 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
191	TGCAPPGKNIYYKSD	ubiquitination	[1]
196	PGKNIYYKSDLTKDI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. 

Sequence Annotation

 REGION 117 118 S-adenosyl-L-methionine binding.
 BINDING 22 22 S-adenosyl-L-methionine.
 BINDING 31 31 S-adenosyl-L-methionine.
 BINDING 34 34 Substrate.
 BINDING 41 41 S-adenosyl-L-methionine.
 BINDING 65 65 S-adenosyl-L-methionine; via carbonyl
 BINDING 86 86 S-adenosyl-L-methionine.
 BINDING 137 137 S-adenosyl-L-methionine; via carbonyl
 BINDING 139 139 Substrate.
 BINDING 176 176 Substrate.
 BINDING 221 221 Substrate.
 MOD_RES 2 2 N-acetylvaline.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Folate-binding; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 293 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0005542; F:folic acid binding; IDA:RGD.
 GO:0016594; F:glycine binding; IDA:UniProtKB.
 GO:0017174; F:glycine N-methyltransferase activity; IDA:UniProtKB.
 GO:0046655; P:folic acid metabolic process; TAS:RGD.
 GO:0006544; P:glycine metabolic process; IMP:RGD.
 GO:0051289; P:protein homotetramerization; IPI:UniProtKB.
 GO:0046498; P:S-adenosylhomocysteine metabolic process; IDA:RGD.
 GO:0046500; P:S-adenosylmethionine metabolic process; IDA:UniProtKB.
 GO:1901052; P:sarcosine metabolic process; IMP:RGD. 

Interpro

 IPR014369; Gly/Sar_N_MeTrfase. 

Pfam

  

SMART

  

PROSITE

 PS51600; SAM_GNMT 

PRINTS