UniProt Accession

 EPN4_HUMAN; Q14677; B7Z6F8; D3DQJ6; Q8NAF1; Q96E05 

Genbank Protein ID

 BK000414; AF434813; D79993; AK092765; AK300257; AC011394; AC026407; CH471062; CH471062; BC004467; BC013091 

Genbank Nucleotide ID

 DAA00062.1; AAL30768.1; BAA11488.2; BAC03971.1; BAH13244.1; EAW61585.1; EAW61588.1; AAH04467.1; AAH13091.1 

Protein Name

 Clathrin interactor 1 

Protein Synonyms/Alias

 Clathrin-interacting protein localized in the trans-Golgi region; Clint; Enthoprotin; Epsin-4; Epsin-related protein; EpsinR 

Gene Name

 CLINT1 

Gene Synonyms/Alias

 ENTH; EPN4; EPNR; KIAA0171 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MLNMWKVRELVDK	ubiquitination	[1, 2]
13	KVRELVDKATNVVMN	ubiquitination	[3, 4]
122	HFVDEHGKDQGINIR	ubiquitination	[2, 4, 5, 6]
133	INIRQKVKELVEFAQ	ubiquitination	[4]
156	RKKAKKNKDKYVGVS	ubiquitination	[2, 4]
158	KAKKNKDKYVGVSSD	ubiquitination	[2, 4, 7]
181	ERYDPEPKSKWDEEW	ubiquitination	[2]
183	YDPEPKSKWDEEWDK	ubiquitination	[1, 2, 4, 5, 6, 7]
190	KWDEEWDKNKSAFPF	ubiquitination	[1, 2, 3, 4, 6]
192	DEEWDKNKSAFPFSD	ubiquitination	[2, 3, 4, 6]
200	SAFPFSDKLGELSDK	ubiquitination	[2, 3, 4, 5, 6, 7]
207	KLGELSDKIGSTIDD	ubiquitination	[1, 2, 3, 4, 5, 6]
218	TIDDTISKFRRKDRE	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
247	ARRGRSPKGEFKDEE	ubiquitination	[2, 3, 4, 5, 6]
251	RSPKGEFKDEEETVT	ubiquitination	[2, 3]
260	EEETVTTKHIHITQA	ubiquitination	[5]
283	KRTANPSKTIDLGAA	acetylation	[4, 8]
283	KRTANPSKTIDLGAA	ubiquitination	[2, 4, 5]
297	AAHYTGDKASPDQNA	ubiquitination	[1, 2, 3, 4, 5]
314	HTPQSSVKTSVPSSK	ubiquitination	[1, 2, 3]
467	QNTDMVQKSVSKTLP	ubiquitination	[1, 2, 3, 6, 7]
471	MVQKSVSKTLPSTWS	ubiquitination	[1, 3, 7]
625	ANFANFSK*******	ubiquitination	[3, 4, 9]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly. 

Sequence Annotation

 DOMAIN 16 149 ENTH.
 BINDING 29 29 Phosphatidylinositol lipid headgroup (By
 BINDING 67 67 Phosphatidylinositol lipid headgroup (By
 MOD_RES 163 163 Phosphoserine (By similarity).
 MOD_RES 227 227 Phosphoserine.
 MOD_RES 232 232 Phosphoserine (By similarity).
 MOD_RES 234 234 Phosphoserine (By similarity).
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 625 AA 

Protein Sequence

Gene Ontology

 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome. 

Interpro

 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N. 

Pfam

 PF01417; ENTH 

SMART

 SM00273; ENTH 

PROSITE

 PS50942; ENTH 

PRINTS