CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005156
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Casein kinase I homolog 1 
Protein Synonyms/Alias
  
Gene Name
 YCK1 
Gene Synonyms/Alias
 CKI2; YHR135C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
103AIKFEPRKTEAPQLRubiquitination[1]
390KSRKHRNKQLQMQQLubiquitination[1]
414QQQQYAQKTEADMRNubiquitination[1, 2]
443YQHQTQQKYLQEQQKubiquitination[1]
450KYLQEQQKRQQQQKLubiquitination[1]
456QKRQQQQKLQEQQLQubiquitination[1]
529SSLAASHKGFFQKLGubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. 
Sequence Annotation
 DOMAIN 69 353 Protein kinase.
 NP_BIND 75 83 ATP (By similarity).
 ACT_SITE 188 188 Proton acceptor (By similarity).
 BINDING 98 98 ATP (By similarity).
 MOD_RES 522 522 Phosphoserine.
 MOD_RES 523 523 Phosphoserine.
 MOD_RES 527 527 Phosphoserine.
 LIPID 537 537 S-palmitoyl cysteine (By similarity).
 LIPID 538 538 S-palmitoyl cysteine (By similarity).  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Kinase; Lipoprotein; Membrane; Nucleotide-binding; Palmitate; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 538 AA 
Protein Sequence
MSMPIASTTL AVNNLTNING NANFNVQANK QLHHQAVDSP ARSSMTATTA ANSNSNSSRD 60
DSTIVGLHYK IGKKIGEGSF GVLFEGTNMI NGVPVAIKFE PRKTEAPQLR DEYKTYKILN 120
GTPNIPYAYY FGQEGLHNIL VIDLLGPSLE DLFDWCGRKF SVKTVVQVAV QMITLIEDLH 180
AHDLIYRDIK PDNFLIGRPG QPDANNIHLI DFGMAKQYRD PKTKQHIPYR EKKSLSGTAR 240
YMSINTHLGR EQSRRDDMEA LGHVFFYFLR GHLPWQGLKA PNNKQKYEKI GEKKRSTNVY 300
DLAQGLPVQF GRYLEIVRSL SFEECPDYEG YRKLLLSVLD DLGETADGQY DWMKLNDGRG 360
WDLNINKKPN LHGYGHPNPP NEKSRKHRNK QLQMQQLQMQ QLQQQQQQQQ YAQKTEADMR 420
NSQYKPKLDP TSYEAYQHQT QQKYLQEQQK RQQQQKLQEQ QLQEQQLQQQ QQQQQQLRAT 480
GQPPSQPQAQ TQSQQFGARY QPQQQPSAAL RTPEQHPNDD NSSLAASHKG FFQKLGCC 538 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0000902; P:cell morphogenesis; IGI:SGD.
 GO:0000910; P:cytokinesis; IGI:SGD.
 GO:0006897; P:endocytosis; IGI:SGD.
 GO:0009749; P:response to glucose stimulus; IMP:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS