UniProt Accession

 ENT1_YEAST; Q12518; D6VRI8 

Genbank Protein ID

 Z74210; Z67750; BK006938 

Genbank Nucleotide ID

 CAA98736.1; CAA91585.1; DAA11698.1 

Protein Name

 Epsin-1 

Protein Synonyms/Alias

  

Gene Name

 ENT1 

Gene Synonyms/Alias

 YDL161W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
103	RENLYIIKTLKEFRH	ubiquitination	[1]
148	EERNMNIKGRNRKGR	ubiquitination	[1]
189	EEDERRRKQDEDYET	ubiquitination	[1]
336	PQQLQQQKQQEPLIQ	ubiquitination	[1]
354	GNQSMTDKYSKLNEL	ubiquitination	[1]
357	SMTDKYSKLNELLAT	ubiquitination	[1, 2]
385	RIPAQHTKTGTFINS	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Binds to membranes enriched in phosphatidylinositol 3,5- bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation. 

Sequence Annotation

 DOMAIN 11 143 ENTH.
 REPEAT 165 184 UIM 1.
 REPEAT 189 208 UIM 2.
 REGION 447 454 Clathrin-binding.
 MOD_RES 160 160 Phosphothreonine.
 MOD_RES 163 163 Phosphoserine.
 MOD_RES 180 180 Phosphothreonine.
 MOD_RES 328 328 Phosphoserine.
 MOD_RES 364 364 Phosphothreonine.
 MOD_RES 366 366 Phosphothreonine.
 MOD_RES 384 384 Phosphothreonine.
 MOD_RES 386 386 Phosphothreonine.
 MOD_RES 388 388 Phosphothreonine.
 MOD_RES 395 395 Phosphothreonine; by PRK1.
 MOD_RES 415 415 Phosphothreonine; by PRK1.  

Keyword

 Complete proteome; Cytoplasm; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 454 AA 

Protein Sequence

Gene Ontology

 GO:0030479; C:actin cortical patch; TAS:SGD.
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0030276; F:clathrin binding; TAS:SGD.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0000147; P:actin cortical patch assembly; IMP:SGD.
 GO:0007015; P:actin filament organization; IMP:SGD.
 GO:0006897; P:endocytosis; IMP:SGD. 

Interpro

 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N.
 IPR003903; Ubiquitin-int_motif. 

Pfam

 PF01417; ENTH 

SMART

 SM00273; ENTH
 SM00726; UIM 

PROSITE

 PS50942; ENTH
 PS50330; UIM 

PRINTS