CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021906
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glyceraldehyde 3-phosphate dehydrogenase GAPA2, chloroplastic 
Protein Synonyms/Alias
 NADP-dependent glyceraldehydephosphate dehydrogenase A subunit 2 
Gene Name
 GAPA2 
Gene Synonyms/Alias
 At1g12900; F13K23.15 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
314DLVVQVSKKTFAEEVacetylation[1]
Reference
 [1] Proteins of diverse function and subcellular location are lysine acetylated in Arabidopsis.
 Finkemeier I, Laxa M, Miguet L, Howden AJ, Sweetlove LJ.
 Plant Physiol. 2011 Apr;155(4):1779-90. [PMID: 21311031
Functional Description
 Involved in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). Catalyzes the reduction of 1,3-diphophoglycerate by NADPH (By similarity). 
Sequence Annotation
 NP_BIND 74 75 NADP (By similarity).
 REGION 215 217 Glyceraldehyde 3-phosphate binding (By
 REGION 274 275 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 216 216 Nucleophile (By similarity).
 BINDING 98 98 NADP (By similarity).
 BINDING 143 143 NADP; via carbonyl oxygen (By
 BINDING 246 246 Glyceraldehyde 3-phosphate (By
 BINDING 297 297 Glyceraldehyde 3-phosphate (By
 BINDING 379 379 NADP (By similarity).  
Keyword
 Alternative splicing; Calvin cycle; Chloroplast; Complete proteome; NADP; Oxidoreductase; Plastid; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 399 AA 
Protein Sequence
MASATFSVAK PSLQGFSEFS GLRNSSALPF AKRSSSDEFV SFVSFQTSAM RSNGGYRKGV 60
TEAKIKVAIN GFGRIGRNFL RCWHGRKDSP LDVVVINDTG GVKQASHLLK YDSTLGIFDA 120
DVKPSGDSAL SVDGKIIKIV SDRNPSNLPW GELGIDLVIE GTGVFVDRDG AGKHLQAGAK 180
KVLITAPGKG DIPTYVVGVN AELYSHEDTI ISNASCTTNC LAPFVKVLDQ KFGIIKGTMT 240
TTHSYTGDQR LLDASHRDLR RARAAALNIV PTSTGAAKAV ALVLPNLKGK LNGIALRVPT 300
PNVSVVDLVV QVSKKTFAEE VNAAFRDAAE KELKGILDVC DEPLVSVDFR CSDVSSTIDS 360
SLTMVMGDDM VKVIAWYDNE WGYSQRVVDL ADIVANNWK 399 
Gene Ontology
 GO:0048046; C:apoplast; IDA:TAIR.
 GO:0009941; C:chloroplast envelope; IDA:TAIR.
 GO:0009570; C:chloroplast stroma; IDA:TAIR.
 GO:0016020; C:membrane; IDA:TAIR.
 GO:0047100; F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006006; P:glucose metabolic process; IEA:InterPro.
 GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-UniPathway. 
Interpro
 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 
SMART
 SM00846; Gp_dh_N 
PROSITE
 PS00071; GAPDH 
PRINTS
 PR00078; G3PDHDRGNASE.