CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019084
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peptidylprolyl isomerase domain and WD repeat-containing protein 1 
Protein Synonyms/Alias
 Spliceosome-associated cyclophilin 
Gene Name
 PPWD1 
Gene Synonyms/Alias
 KIAA0073 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
65ATLAKKRKVLEFERVubiquitination[1]
127EEGIEFVKHFRSHLGubiquitination[2, 3]
220QPLHIFDKLHTSPLTubiquitination[1]
278TDLYEFAKCKAYPTSubiquitination[1]
394RCVRILGKQENIRVMubiquitination[1]
444TIVCTSFKKNRFYMFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. 
Sequence Annotation
 REPEAT 88 126 WD 1.
 REPEAT 131 170 WD 2.
 REPEAT 221 260 WD 3.
 REPEAT 278 319 WD 4.
 DOMAIN 490 645 PPIase cyclophilin-type.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Isomerase; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Rotamase; Spliceosome; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 646 AA 
Protein Sequence
MAAESGSDFQ QRRRRRRDPE EPEKTELSER ELAVAVAVSQ ENDEENEERW VGPLPVEATL 60
AKKRKVLEFE RVYLDNLPSA SMYERSYMHR DVITHVVCTK TDFIITASHD GHVKFWKKIE 120
EGIEFVKHFR SHLGVIESIA VSSEGALFCS VGDDKAMKVF DVVNFDMINM LKLGYFPGQC 180
EWIYCPGDAI SSVAASEKST GKIFIYDGRG DNQPLHIFDK LHTSPLTQIR LNPVYKAVVS 240
SDKSGMIEYW TGPPHEYKFP KNVNWEYKTD TDLYEFAKCK AYPTSVCFSP DGKKIATIGS 300
DRKVRIFRFV TGKLMRVFDE SLSMFTELQQ MRQQLPDMEF GRRMAVEREL EKVDAVRLIN 360
IVFDETGHFV LYGTMLGIKV INVETNRCVR ILGKQENIRV MQLALFQGIA KKHRAATTIE 420
MKASENPVLQ NIQADPTIVC TSFKKNRFYM FTKREPEDTK SADSDRDVFN EKPSKEEVMA 480
ATQAEGPKRV SDSAIIHTSM GDIHTKLFPV ECPKTVENFC VHSRNGYYNG HTFHRIIKGF 540
MIQTGDPTGT GMGGESIWGG EFEDEFHSTL RHDRPYTLSM ANAGSNTNGS QFFITVVPTP 600
WLDNKHTVFG RVTKGMEVVQ RISNVKVNPK TDKPYEDVSI INITVK 646 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IEA:UniProtKB-KW.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB.
 GO:0006457; P:protein folding; IEA:UniProtKB-KW.
 GO:0000413; P:protein peptidyl-prolyl isomerization; IEA:GOC. 
Interpro
 IPR002130; Cyclophilin-like_PPIase_dom.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00160; Pro_isomerase
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00170; CSA_PPIASE_1
 PS50072; CSA_PPIASE_2
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00153; CSAPPISMRASE.