UniProt Accession

 3BP5_HUMAN; O60239; B3KQW6; Q5JWV9 

Genbank Protein ID

 AK090524; AC087590; AL117422; CH471055; BC010123; AB005047 

Genbank Nucleotide ID

 BAG52178.1; CAI46220.1; EAW64225.1; AAH10123.2; BAA25922.1 

Protein Name

 SH3 domain-binding protein 5 

Protein Synonyms/Alias

 SH3BP-5; SH3 domain-binding protein that preferentially associates with BTK 

Gene Name

 SH3BP5 

Gene Synonyms/Alias

 SAB 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MDAALKRSRSEEP	ubiquitination	[1]
51	RIQGELEKLNQSTDD	ubiquitination	[1]
93	ELVKKIGKAVEDSKP	ubiquitination	[1]
99	GKAVEDSKPYWEARR	ubiquitination	[1]
117	QAQLEAQKATQDFQR	ubiquitination	[1, 2, 3]
133	TEVLRAAKETISLAE	ubiquitination	[1, 3]
173	VMEAEQTKTRSELVH	ubiquitination	[1]
181	TRSELVHKETAARYN	ubiquitination	[1]
209	KRAINKSKPYFELKA	ubiquitination	[1]
215	SKPYFELKAKYYVQL	ubiquitination	[1, 2, 4]
217	PYFELKAKYYVQLEQ	ubiquitination	[1]
227	VQLEQLKKTVDDLQA	ubiquitination	[1]
240	QAKLTLAKGEYKMAL	ubiquitination	[1]
244	TLAKGEYKMALKNLE	ubiquitination	[1]
248	GEYKMALKNLEMISD	ubiquitination	[1, 2, 4, 5, 6]
393	RAEGAENKTSDKANN	ubiquitination	[4]
415	SGSGGSSKSQSSTSP	ubiquitination	[1, 2, 4, 7]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death. 

Sequence Annotation

 MOD_RES 351 351 Phosphoserine; by MAPK12 and MAPK9 (By
 MOD_RES 418 418 Phosphoserine (By similarity).
 MOD_RES 421 421 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Mitochondrion; Phosphoprotein; Reference proteome; SH3-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 455 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0004860; F:protein kinase inhibitor activity; IDA:MGI.
 GO:0035556; P:intracellular signal transduction; IDA:MGI. 

Interpro

 IPR007940; SH3-bd_5. 

Pfam

 PF05276; SH3BP5 

SMART

  

PROSITE

  

PRINTS