CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037387
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 DLAT 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
271GIDLTQVKGTGPDGRubiquitination[1]
282PDGRITKKDIDSFVPacetylation[2]
361LVRKELNKILEGRSKacetylation[2, 3, 4]
361LVRKELNKILEGRSKubiquitination[5]
368KILEGRSKISVNDFIubiquitination[1, 5]
442DVVSLATKAREGKLQubiquitination[1, 2, 5]
536EFRKYLEKPITMLL*ubiquitination[5]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Acyltransferase; Complete proteome; Lipoyl; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 542 AA 
Protein Sequence
MWRVCARRAQ NVAPWAGLEA RWTALQEVPG TPRVTSRSGP APARRNSVTT GYGGVRALCG 60
WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS PTMQAGTIAR WEKKEGDKIN 120
EGDLIAEVET DKATVGFESL EECYMAKILV AEGTRDVPIG AIICITVGKP EDIEAFKNYT 180
LDSSAAPTPQ AAPAPTPAAT ASPPTPSAQA PGSSYPPHMQ VAAVPPTPQP LAPTPSAPCP 240
ATPAGPKGRV FVSPLAKKLA VEKGIDLTQV KGTGPDGRIT KKDIDSFVPS KVAPAPAAVV 300
PPTGPGMAPV PTGVFTDIPI SNIRRVIAQR LMQSKQTIPH YYLSIDVNMG EVLLVRKELN 360
KILEGRSKIS VNDFIIKASA LACLKVPEAN SSWMDTVIRQ NHVVDVSVAV STPAGLITPI 420
VFNAHIKGVE TIANDVVSLA TKAREGKLQP HEFQGGTFTI SNLGMFGIKN FSAIINPPQA 480
CILAIGASED KLVPADNEKG FDVASMMSVT LSCDHRVVDG AVGAQWLAEF RKYLEKPITM 540
LL 542 
Gene Ontology
 GO:0045254; C:pyruvate dehydrogenase complex; IEA:InterPro.
 GO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IEA:InterPro.
 GO:0006090; P:pyruvate metabolic process; IEA:InterPro. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR004167; E3-bd.
 IPR006257; LAT1.
 IPR011053; Single_hybrid_motif. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl
 PF02817; E3_binding 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS