CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007088
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Palmitoyltransferase AKR1 
Protein Synonyms/Alias
 Ankyrin repeat-containing protein AKR1 
Gene Name
 AKR1 
Gene Synonyms/Alias
 YDR264C; D9954.9; YD9230B.03C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
484IRKPLRSKFSPLNNAacetylation[1]
484IRKPLRSKFSPLNNAubiquitination[2]
755KLYKLPNKDVEQGNDubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Palmitoyltransferase specific for casein kinase 1. Palmitoylates isoforms YCK1 and YCK2 at both C-terminal cysteine residues, which is required for their proper plasma membrane localization. Required for constitutive endocytosis of a-factor receptor STE3 and both constitutive and pheromone-induced endocytosis of alpha-factor receptor STE2. 
Sequence Annotation
 REPEAT 72 102 ANK 1.
 REPEAT 108 137 ANK 2.
 REPEAT 142 171 ANK 3.
 REPEAT 175 204 ANK 4.
 REPEAT 213 242 ANK 5.
 REPEAT 246 275 ANK 6.
 ZN_FING 470 520 DHHC-type.
 ACT_SITE 500 500 S-palmitoyl cysteine intermediate
 MOD_RES 51 51 Phosphoserine.
 MOD_RES 57 57 Phosphoserine.  
Keyword
 Acyltransferase; ANK repeat; Complete proteome; Endosome; Golgi apparatus; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Transferase; Transmembrane; Transmembrane helix; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 764 AA 
Protein Sequence
MVNELENVPR ASTLTNEEQT VDPSNNDSQE DISLGDSNEI TSLASLKAIR SGNEEESGNE 60
QVNHNDEAEE DPLLTRYHTA CQRGDLATVK EMIHGKLLEV NNDGDSTEHI TGLHWASINN 120
RLSVVDFLVS QGADVNARAG ALHATPLHWA ARYGYVYIVD FLLKHGADPT MTDDQGFNLL 180
HLSVNSSNIM LVLYVLFNVV SKGLLDIDCR DPKGRTSLLW AAYQGDSLTV AELLKFGASI 240
KIADTEGFTP LHWGTVKGQP HVLKYLIQDG ADFFQKTDTG KDCFAIAQEM NTVYSLREAL 300
THSGFDYHGY PIKKWFKKSQ HAKLVTFITP FLFLGIAFAL FSHINPLFVI IVLFLLAIAT 360
NKGLNKFVLP SYGRMGVHNV TLLRSPLLSG VFFGTLLWVT IVWFFKVMPR TFSDEQYTNI 420
LMLVILVSVF YLFGQLVIMD PGCLPEETDH ENVRQTISNL LEIGKFDTKN FCIETWIRKP 480
LRSKFSPLNN AVVARFDHYC PWIFNDVGLK NHKAFIFFIT LMESGIFTFL ALCLEYFDEL 540
EDAHEDTSQK NGKCFILGAS DLCSGLIYDR FVFLILLWAL LQSIWVASLI FVQAFQICKG 600
MTNTEFNVLM KESKSIGPDG LSFNENFNTT PEGFAPSIDP GEESNDTVLA PVPGSTIRKP 660
RTCFGVCYAV TGMDQWLAVI KETIGIKDST GHNVYSITSR IPTNYGWKRN VKDFWLTSDI 720
NAPLWRRILY PPSGSKALLN GIEVDYFKLY KLPNKDVEQG NDMV 764 
Gene Ontology
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0016409; F:palmitoyltransferase activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0018345; P:protein palmitoylation; IDA:SGD.
 GO:0006612; P:protein targeting to membrane; IMP:SGD.
 GO:0030100; P:regulation of endocytosis; IMP:SGD.
 GO:0010969; P:regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion; IGI:SGD. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR001594; Znf_DHHC_palmitoyltrfase. 
Pfam
 PF00023; Ank
 PF01529; zf-DHHC 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50216; ZF_DHHC 
PRINTS