CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031342
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA replication licensing factor MCM3 
Protein Synonyms/Alias
 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), isoform CRA_b; cDNA FLJ55599, highly similar to DNA replication licensing factor MCM3 
Gene Name
 MCM3 
Gene Synonyms/Alias
 hCG_22498 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80DQGIYQSKVRELISDubiquitination[1, 2, 3, 4, 5, 6]
126VAFQRALKDFVASIDubiquitination[4]
185KCSLVRPKVVRSVHYubiquitination[4]
252TIQEMPEKAPAGQLPubiquitination[1]
273LDDDLVDKAKPGDRVubiquitination[4]
275DDLVDKAKPGDRVQVubiquitination[4]
293YRCLPGKKGGYTSGTubiquitination[4, 6]
311VLIACNVKQMSKDAQubiquitination[3, 4, 6, 7, 8, 9]
315CNVKQMSKDAQPSFSubiquitination[4]
338KFSKTRSKDIFDQLAubiquitination[4, 6, 7, 8, 9]
346DIFDQLAKSLAPSIHubiquitination[7, 9]
360HGHDYVKKAILCLLLubiquitination[4]
396IGDPSVAKSQLLRYVubiquitination[1, 2, 6, 7, 9, 10]
458VCIDEFDKMSDMDRTubiquitination[4, 6]
480QGRVTIAKAGIHARLubiquitination[2, 3, 4, 5, 6, 7, 9, 10]
508YGRYDQYKTPMENIGubiquitination[1, 2, 4, 5, 6]
615VSAAFMKKYIHVAKIubiquitination[4]
624IHVAKIIKPVLTQESubiquitination[7, 9]
777TADSQETKESQKVELubiquitination[1, 4]
781QETKESQKVELSESRacetylation[11]
781QETKESQKVELSESRubiquitination[4]
793ESRLKAFKVALLDVFubiquitination[7, 9]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [11] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; DNA replication; DNA-binding; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 853 AA 
Protein Sequence
MLPRSPPLPR GNLWWREEFG SFRAGVESSW EPPRDFGGGS SLAAGMAGTV VLDDVELREA 60
QRDYLDFLDD EEDQGIYQSK VRELISDNQY RLIVNVNDLR RKNEKRANRL LNNAFEELVA 120
FQRALKDFVA SIDATYAKQY EEFYVGLEGS FGSKHVSPRT LTSCFLSCVV CVEGIVTKCS 180
LVRPKVVRSV HYCPATKKTI ERRYSDLTTL VAFPSSSVYP TKDEENNPLE TEYGLSVYKD 240
HQTITIQEMP EKAPAGQLPR SVDVILDDDL VDKAKPGDRV QVVGTYRCLP GKKGGYTSGT 300
FRTVLIACNV KQMSKDAQPS FSAEDIAKIK KFSKTRSKDI FDQLAKSLAP SIHGHDYVKK 360
AILCLLLGGV ERDLENGSHI RGDINILLIG DPSVAKSQLL RYVLCTAPRA IPTTGRGSSG 420
VGLTAAVTTD QETGERRLEA GAMVLADRGV VCIDEFDKMS DMDRTAIHEV MEQGRVTIAK 480
AGIHARLNAR CSVLAAANPV YGRYDQYKTP MENIGLQDSL LSRFDLLFIM LDQMDPEQDR 540
EISDHVLRMH RYRAPGEQDG DAMPLGSAVD ILATDDPNFS QEDQQDTQIY EKHDNLLHGT 600
KKKKEKMVSA AFMKKYIHVA KIIKPVLTQE SATYIAEEYS RLRSQDSMSS DTARTSPVTA 660
RTLETLIRLA TAHAKARMSK TVDLQDAEEA VELVQYAYFK KVLEKEKKRK KRSEDESETE 720
DEEEKSQEDQ EQKRKRRKTR QPDAKDGDSY DPYDFSDTEE EMPQVHTPKT ADSQETKESQ 780
KVELSESRLK AFKVALLDVF REAHAQSIGM NRLTESINRD SEEPFSSVEI QAALSKMQDD 840
NQVMVSEGII FLI 853 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0042555; C:MCM complex; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003678; F:DNA helicase activity; IEA:InterPro.
 GO:0032508; P:DNA duplex unwinding; IEA:GOC.
 GO:0006270; P:DNA replication initiation; IEA:InterPro. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR008046; Mcm3.
 IPR018525; MCM_CS.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00493; MCM 
SMART
 SM00382; AAA
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.
 PR01659; MCMPROTEIN3.