CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005523
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leucine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Leucyl-tRNA synthetase; LeuRS 
Gene Name
 CDC60 
Gene Synonyms/Alias
 YPL160W; P2564 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
21DALIAIEKKYQKIWAacetylation[1]
126REAELFGKNFDNVPAacetylation[1]
153KDHEDVTKFKAKKSKacetylation[1]
187IPREEIIKFADAKYWacetylation[1]
358FKNMSYQKLTPKRGFacetylation[1]
480KNLLAEAKKIAYKEDacetylation[1]
485EAKKIAYKEDYYTGTacetylation[1]
672IPLPALQKLRREFEYacetylation[1]
672IPLPALQKLRREFEYubiquitination[2]
830QYALTNYKNALKYGLubiquitination[2]
856EASGVMHKDLIARYIacetylation[1]
896QTSVQNAKFPRASKPacetylation[1]
896QTSVQNAKFPRASKPubiquitination[2]
1009KQRLANEKPEDVFERacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 MOTIF 66 76 "HIGH" region.
 MOTIF 729 733 "KMSKS" region.
 BINDING 732 732 ATP (By similarity).
 MOD_RES 142 142 Phosphothreonine.  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1090 AA 
Protein Sequence
MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE ELHRTYPKFM 60
SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP LGFHCTGMPI LACADKLKRE 120
AELFGKNFDN VPAEEEEIKE ETPAEKDHED VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI 180
PREEIIKFAD AKYWLTYFPP LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK 240
AAGKIKFGER YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS 300
DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA FKNMSYQKLT 360
PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV IATKGTGVVT CVPSNSPDDY 420
ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK 480
KIAYKEDYYT GTMIYGPYKG EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV 540
SLEDQWYVDY GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP 600
WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF DYIFQHQDDV 660
KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF IYTHVALFPK KFWPKGIRAN 720
GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD AARIAFADAG DTVEDANFDE SNANAAILRL 780
FNLKEWAEEI TKESNLRTGE ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF 840
QAARDYYREA SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA 900
SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL LISESFPEWQ 960
SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL LKQRLANEKP EDVFERELQF 1020
SEIDTVKAAA RNVKKAAQAL KIAEFSAISF PYGAKTGKDI FTGEEVEIPP VTKIVENAVP 1080
GNPGVVFQNI 1090 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004823; F:leucine-tRNA ligase activity; IDA:SGD.
 GO:0006429; P:leucyl-tRNA aminoacylation; IDA:SGD.
 GO:0032006; P:regulation of TOR signaling cascade; IMP:SGD.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 
Interpro
 IPR002300; aa-tRNA-synth_Ia.
 IPR004493; Leu-tRNA-synth_Ia_arc/euk.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR013155; V/L/I-tRNA-synth_anticodon-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit. 
Pfam
 PF08264; Anticodon_1
 PF00133; tRNA-synt_1 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS