UniProt Accession

 ERG5_YEAST; P54781; D6VZI9; E9P965 

Genbank Protein ID

 U34636; Z49211; AY723852; BK006946 

Genbank Nucleotide ID

 AAB06217.1; CAA89116.1; AAU09769.1; DAA09913.1 

Protein Name

 Cytochrome P450 61 

Protein Synonyms/Alias

 C-22 sterol desaturase 

Gene Name

 ERG5 

Gene Synonyms/Alias

 CYP61; YMR015C; YM9711.02C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
97	DPKFEEYKAKWASGP	ubiquitination	[1]
113	SCVSIFHKFVVIAST	ubiquitination	[1]
126	STRDLARKILQSSKF	ubiquitination	[1]
164	KAHTDYRKSLNGLFT	ubiquitination	[2, 3, 4]
192	IMDKYMDKFVRLSKE	ubiquitination	[1]
198	DKFVRLSKENNYEPQ	ubiquitination	[1, 2, 3, 4, 5]
266	TKTWYGKKTADMAMK	ubiquitination	[1]
273	KTADMAMKIFENCAQ	ubiquitination	[1]
283	ENCAQMAKDHIAAGG	ubiquitination	[1]
291	DHIAAGGKPVCVMDA	ubiquitination	[1]
301	CVMDAWCKLMHDAKN	ubiquitination	[1]
307	CKLMHDAKNSNDDDS	ubiquitination	[1, 6]
385	LNLDLIEKMKYTNMV	ubiquitination	[1]
387	LDLIEKMKYTNMVIK	ubiquitination	[1]
412	MVPYVVKKNFPVSPN	ubiquitination	[1]
458	ERWVEGSKASEAKKN	ubiquitination	[1]
519	VFATIFPKDDLLLTF	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [5] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149]
 [6] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Required to form the C-22(23) double bond in the sterol side chain. 

Sequence Annotation

 METAL 476 476 Iron (heme axial ligand) (By similarity).
 CROSSLNK 164 164 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 198 198 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Direct protein sequencing; Heme; Iron; Isopeptide bond; Lipid biosynthesis; Lipid metabolism; Metal-binding; Oxidoreductase; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 538 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0000249; F:C-22 sterol desaturase activity; IDA:SGD.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004497; F:monooxygenase activity; IEA:InterPro.
 GO:0006696; P:ergosterol biosynthetic process; IDA:SGD. 

Interpro

 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002403; Cyt_P450_E_grp-IV. 

Pfam

 PF00067; p450 

SMART

  

PROSITE

 PS00086; CYTOCHROME_P450 

PRINTS

 PR00465; EP450IV.