CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018385
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 6 
Protein Synonyms/Alias
  
Gene Name
 KLHL6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18TMGDVVEKSLEGPLAubiquitination[1]
34STDEPSQKTGDLVEIubiquitination[1]
48ILNGEKVKFDDAGLSubiquitination[1]
106AMFCNDLKEKYEKRIubiquitination[1]
116YEKRIIIKGVDAETMubiquitination[1]
134LDYTYTSKALITKQNubiquitination[1]
139TSKALITKQNVQRVLubiquitination[1, 2, 3]
190THSLDSLKKQVQSYIubiquitination[1]
191HSLDSLKKQVQSYIIubiquitination[1]
485NGKLATDKTQCYDPSubiquitination[1, 2, 4]
495CYDPSTNKWSLKAAMubiquitination[1, 4]
499STNKWSLKAAMPVEAubiquitination[1]
507AAMPVEAKCINAVSFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in B-lymphocyte antigen receptor signaling and germinal center formation (By similarity). 
Sequence Annotation
 DOMAIN 72 139 BTB.
 DOMAIN 174 276 BACK.
 REPEAT 320 367 Kelch 1.
 REPEAT 378 421 Kelch 2.
 REPEAT 422 468 Kelch 3.
 REPEAT 470 516 Kelch 4.
 REPEAT 517 558 Kelch 5.
 REPEAT 560 606 Kelch 6.  
Keyword
 Complete proteome; Kelch repeat; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 621 AA 
Protein Sequence
MLMAGQRGAW TMGDVVEKSL EGPLAPSTDE PSQKTGDLVE ILNGEKVKFD DAGLSLILQN 60
GLETLRMENA LTDVILCVDI QEFSCHRVVL AAASNYFRAM FCNDLKEKYE KRIIIKGVDA 120
ETMHTLLDYT YTSKALITKQ NVQRVLEAAN LFQFLRMVDA CASFLTEALN PENCVGILRL 180
ADTHSLDSLK KQVQSYIIQN FVQILNSEEF LDLPVDTLHH ILKSDDLYVT EEAQVFETVM 240
SWVRHKPSER LCLLPYVLEN VRLPLLDPWY FVETVEADPL IRQCPEVFPL LQEARMYHLS 300
GNEIISERTK PRMHEFQSEV FMIIGGCTKD ERFVAEVTCL DPLRRSRLEV AKLPLTEHEL 360
ESENKKWVEF ACVTLKNEVY ISGGKETQHD VWKYNSSINK WIQIEYLNIG RWRHKMVVLG 420
GKVYVIGGFD GLQRINNVET YDPFHNCWSE AAPLLVHVSS FAATSHKKKL YVIGGGPNGK 480
LATDKTQCYD PSTNKWSLKA AMPVEAKCIN AVSFRDRIYV VGGAMRALYA YSPLEDSWCL 540
VTQLSHERAS CGIAPCNNRL YITGGRDEKN EVIATVLCWD PEAQKLTEEC VLPRGVSHHG 600
SVTIRKSYTH IRRIVPGAVS V 621 
Gene Ontology
 GO:0050853; P:B cell receptor signaling pathway; IEA:Compara.
 GO:0002467; P:germinal center formation; IEA:Compara. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS