CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042510
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform 
Protein Synonyms/Alias
  
Gene Name
 PIK3CB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
343GHMKVLSKQVEALNKubiquitination[1]
364LIKLNAVKLNRAKGKubiquitination[1]
460RLMDLLWKEAGLDLRubiquitination[2]
518DALLNWLKEYNSGDDubiquitination[2]
657VKDIQYLKDSLALGKubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 702 AA 
Protein Sequence
XVRAGLFHGT ELLCKTIVSS EVSGKNDHIW NEPLEFDINI CDLPRMARLC FAVYAVLDKV 60
KTKKSTKTIN PSKYQTIRKA GKVHYPVAWV NTMVFDFKGQ LRTGDIILHS WSSFPDELEE 120
MLNPMGTVQT NPYTENATAL HVKFPENKKQ PYYYPPFDKS RGGKKFLPVL KEILDRDPLS 180
QLCENEMDLI WTLRQDCREI FPQSLPKLLL SIKWNKLEDV AQLQALLQIW PKLPPREALE 240
LLDFNYPDQY VREYAVGCLR QMSDEELSQY LLQLVQVLKY EPFLDCALSR FLLERALGNR 300
RIGQFLFWHL RSEVHIPAVS VQFGVILEAY CRGSVGHMKV LSKQVEALNK LKTLNSLIKL 360
NAVKLNRAKG KEAMHTCLKQ SAYREALSDL QSPLNPCVIL SELYVEKCKY MDSKMKPLWL 420
VYNNKVFGED SVGVIFKNGD DLRQDMLTLQ MLRLMDLLWK EAGLDLRMLP YGCLATGDRS 480
GLIEVVSTSE TIADIQLNSS NVAAAAAFNK DALLNWLKEY NSGDDLDRAI EEFTLSCAGY 540
CVASYVLGIG DRHSDNIMVK KTGQLFHIDF GHILGNFKSK FGIKRERVPF ILTYDFIHVI 600
QQGKTGNTEK FGRFRQCCED AYLILRRHGN LFITLFALML TAGLPELTSV KDIQYLKDSL 660
ALGKSEEEAL KQFKQKFDEA LRESWTTKVN WMAHTVRKDY RS 702 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
 GO:0046854; P:phosphatidylinositol phosphorylation; IEA:InterPro.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR011009; Kinase-like_dom.
 IPR000403; PI3/4_kinase_cat_dom.
 IPR018936; PI3/4_kinase_CS.
 IPR002420; PI3K_C2_dom.
 IPR015433; PI_Kinase.
 IPR001263; PInositide-3_kin_accessory_dom. 
Pfam
 PF00454; PI3_PI4_kinase
 PF00792; PI3K_C2
 PF00613; PI3Ka 
SMART
 SM00142; PI3K_C2
 SM00145; PI3Ka
 SM00146; PI3Kc 
PROSITE
 PS00915; PI3_4_KINASE_1
 PS00916; PI3_4_KINASE_2
 PS50290; PI3_4_KINASE_3
 PS51547; PI3K_C2
 PS51545; PIK_HELICAL 
PRINTS