UniProt Accession

 GCP60_HUMAN; Q9H3P7; B2RB29; Q5VTJ0; Q6P9F1; Q8IZC5; Q8N4D6; Q9H6U3 

Genbank Protein ID

 AB043587; AY150218; AK025520; AK314468; AL592045; CH471098; BC034563; BC045533; BC060792 

Genbank Nucleotide ID

 BAB20592.2; AAN60219.1; BAB15159.1; BAG37076.1; CAH71922.1; EAW69775.1; AAH34563.2; AAH45533.1; AAH60792.1 

Protein Name

 Golgi resident protein GCP60 

Protein Synonyms/Alias

 Acyl-CoA-binding domain-containing protein 3; Golgi complex-associated protein 1; GOCAP1; Golgi phosphoprotein 1; GOLPH1; PBR- and PKA-associated protein 7; Peripheral benzodiazepine receptor-associated protein PAP7 

Gene Name

 ACBD3 

Gene Synonyms/Alias

 GCP60; GOCAP1; GOLPH1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
163	DAMVEFVKLLNRCCH	ubiquitination	[1, 2]
347	THTDSSEKELEPEAA	acetylation	[3]
386	QIKDFKEKIQQDADS	ubiquitination	[4, 5, 6, 7, 8, 9]
507	GRGVYLLKFDNSYSL	ubiquitination	[1, 2, 4, 6, 7, 8, 9, 10, 11]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity). 

Sequence Annotation

 DOMAIN 83 174 ACB.
 DOMAIN 384 526 GOLD.
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 20 20 Phosphoserine.
 MOD_RES 43 43 Phosphoserine.  

Keyword

 Coiled coil; Complete proteome; Direct protein sequencing; Golgi apparatus; Lipid biosynthesis; Lipid metabolism; Membrane; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Steroid biosynthesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 528 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0000062; F:fatty-acyl-CoA binding; IEA:InterPro.
 GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
 GO:0006810; P:transport; IEA:InterPro. 

Interpro

 IPR022408; Acyl-CoA-binding_prot_CS.
 IPR000582; Acyl-CoA-binding_protein.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR009038; GOLD. 

Pfam

 PF00887; ACBP
 PF13897; GOLD_2 

SMART

  

PROSITE

 PS00880; ACB_1
 PS51228; ACB_2
 PS50866; GOLD 

PRINTS