CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004774
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TFIIH basal transcription factor complex helicase XPB subunit 
Protein Synonyms/Alias
 Basic transcription factor 2 89 kDa subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH 89 kDa subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein 
Gene Name
 ERCC3 
Gene Synonyms/Alias
 XPB; XPBC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
52QVDESGTKVDEYGAKubiquitination[1]
59KVDEYGAKDYRLQMPubiquitination[1]
222TSKSAISKTAESSGGubiquitination[1, 2]
577EYAIRLNKPYIYGPTubiquitination[1]
597MQILQNFKHNPKINTubiquitination[1]
601QNFKHNPKINTIFISubiquitination[3, 4]
706EDLAFSTKEEQQQLLubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. 
Sequence Annotation
 DOMAIN 327 488 Helicase ATP-binding.
 DOMAIN 542 702 Helicase C-terminal.
 NP_BIND 340 347 ATP (By similarity).
 MOTIF 6 18 Nuclear localization signal (Potential).
 MOTIF 441 444 DEVH box.  
Keyword
 3D-structure; ATP-binding; Cockayne syndrome; Complete proteome; Deafness; Disease mutation; DNA damage; DNA repair; DNA-binding; Dwarfism; Helicase; Host-virus interaction; Hydrolase; Ichthyosis; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation; Xeroderma pigmentosum. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 782 AA 
Protein Sequence
MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG TKVDEYGAKD 60
YRLQMPLKDD HTSRPLWVAP DGHIFLEAFS PVYKYAQDFL VAIAEPVCRP THVHEYKLTA 120
YSLYAAVSVG LQTSDITEYL RKLSKTGVPD GIMQFIKLCT VSYGKVKLVL KHNRYFVESC 180
HPDVIQHLLQ DPVIRECRLR NSEGEATELI TETFTSKSAI SKTAESSGGP STSRVTDPQG 240
KSDIPMDLFD FYEQMDKDEE EEEETQTVSF EVKQEMIEEL QKRCIHLEYP LLAEYDFRND 300
SVNPDINIDL KPTAVLRPYQ EKSLRKMFGN GRARSGVIVL PCGAGKSLVG VTAACTVRKR 360
CLVLGNSAVS VEQWKAQFKM WSTIDDSQIC RFTSDAKDKP IGCSVAISTY SMLGHTTKRS 420
WEAERVMEWL KTQEWGLMIL DEVHTIPAKM FRRVLTIVQA HCKLGLTATL VREDDKIVDL 480
NFLIGPKLYE ANWMELQNNG YIAKVQCAEV WCPMSPEFYR EYVAIKTKKR ILLYTMNPNK 540
FRACQFLIKF HERRNDKIIV FADNVFALKE YAIRLNKPYI YGPTSQGERM QILQNFKHNP 600
KINTIFISKV GDTSFDLPEA NVLIQISSHG GSRRQEAQRL GRVLRAKKGM VAEEYNAFFY 660
SLVSQDTQEM AYSTKRQRFL VDQGYSFKVI TKLAGMEEED LAFSTKEEQQ QLLQKVLAAT 720
DLDAEEEVVA GEFGSRSSQA SRRFGTMSSM SGADDTVYME YHSSRSKAPS KHVHPLFKRF 780
RK 782 
Gene Ontology
 GO:0005675; C:holo TFIIH complex; IDA:UniProtKB.
 GO:0000441; C:SSL2-core TFIIH complex; IEA:Compara.
 GO:0043138; F:3'-5' DNA helicase activity; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:Compara.
 GO:0016887; F:ATPase activity; IDA:UniProtKB.
 GO:0003684; F:damaged DNA binding; NAS:UniProtKB.
 GO:0032564; F:dATP binding; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:Compara.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0008134; F:transcription factor binding; IDA:MGI.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0000075; P:cell cycle checkpoint; IMP:UniProtKB.
 GO:0006265; P:DNA topological change; IMP:UniProtKB.
 GO:0035315; P:hair cell differentiation; IMP:UniProtKB.
 GO:0006917; P:induction of apoptosis; IDA:UniProtKB.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0000717; P:nucleotide-excision repair, DNA duplex unwinding; IMP:UniProtKB.
 GO:0033683; P:nucleotide-excision repair, DNA incision; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0008104; P:protein localization; IMP:UniProtKB.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IMP:UniProtKB.
 GO:0009411; P:response to UV; IMP:UniProtKB.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; IDA:UniProtKB.
 GO:0009650; P:UV protection; IEA:Compara.
 GO:0022415; P:viral reproductive process; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR006935; Helicase/UvrB_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR001161; Helicase_Ercc3.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00271; Helicase_C
 PF04851; ResIII 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS
 PR00851; XRODRMPGMNTB.