CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024514
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Putative COBW domain-containing protein 7 
Protein Synonyms/Alias
 Putative cobalamin synthase W domain-containing protein 7 
Gene Name
 CBWD7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28VDSKYGLKHLTEEKPubiquitination[1, 2, 3, 4]
34LKHLTEEKPDGLINEubiquitination[1, 2]
66LVPEEDVKKLRTTIRubiquitination[1, 2, 3]
67VPEEDVKKLRTTIRSubiquitination[2]
110LSGISLQKKLQHVPGubiquitination[1, 3]
158WEKNVRNKDNHCMEVubiquitination[2, 4]
169CMEVIRLKGLVSIKDubiquitination[2]
175LKGLVSIKDKSQQVIubiquitination[2]
177GLVSIKDKSQQVIVQubiquitination[1]
200EETPVSWKDDTERTNubiquitination[1, 2, 3, 5]
218LIGRNLDKDILKQLFubiquitination[2]
222NLDKDILKQLFIATVubiquitination[2, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
 DOMAIN 126 229 CobW C-terminal.
 NP_BIND 55 58 ATP (Potential).  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 247 AA 
Protein Sequence
MFWVDAELGS DIYLDGIITI VDSKYGLKHL TEEKPDGLIN EATRQVALAD IILINKTDLV 60
PEEDVKKLRT TIRSINGLGQ ILETQRSRVD LSNVLDLHAF DSLSGISLQK KLQHVPGTQP 120
HLDQSIVTIT FEVPGNAKEE HLNMFIQNLL WEKNVRNKDN HCMEVIRLKG LVSIKDKSQQ 180
VIVQGVHELY DLEETPVSWK DDTERTNRLV LIGRNLDKDI LKQLFIATVT ETEKQWTTHF 240
KEDQVCT 247 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW. 
Interpro
 IPR011629; Cbl_biosynth_CobW-like_C.
 IPR003495; CobW/HypB/UreG_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF02492; cobW
 PF07683; CobW_C 
SMART
 SM00833; CobW_C 
PROSITE
  
PRINTS