UniProt Accession

 MTO1_HUMAN; Q9Y2Z2; B3KQB5; Q5SWL2; Q5SWL3; Q5SWL4; Q8NDN7; Q8WZ57; Q96FE6; Q9BS06 

Genbank Protein ID

 AF469110; AF469111; AF442963; AF319422; AY078986; AY078985; AF132937; AK074625; AK225828; AL603910; AL603910; AL603910; CH471051; BC005808; BC011051; AL833823 

Genbank Nucleotide ID

 AAL82394.1; AAL82395.1; AAL35894.1; AAG42814.3; AAL85491.1; AAL85490.1; AAD27712.1; BAG51977.1; CAI14880.1; CAI14881.1; CAI14882.1; EAW48762.1; AAH05808.2; AAH11051.2; CAD38685.1 

Protein Name

 Protein MTO1 homolog, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 MTO1 

Gene Synonyms/Alias

 CGI-02 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
227	GLAQTLEKLGFVVGR	ubiquitination	[1, 2, 3]
245	GTPPRIAKESINFSI	ubiquitination	[2, 4, 5]
275	TNETVWIKPEDQLPC	ubiquitination	[1]
297	RVDEIVLKNLHLNSH	ubiquitination	[1, 2, 4, 5, 6]
306	LHLNSHVKETTRGPR	ubiquitination	[2, 5]
321	YCPSIESKVLRFPNR	ubiquitination	[2, 5]
460	ASLRVSRKPPFVVSR	ubiquitination	[2, 5]
555	FLSSKWKKLIPEASI	ubiquitination	[6]
577	VRALDVLKYEEVDMD	ubiquitination	[1]
588	VDMDSLAKAVPEPLK	ubiquitination	[1]
625	FHQLQEIKGVQQDEA	ubiquitination	[1]
637	DEALQLPKDLDYLTI	ubiquitination	[1, 2, 3, 5]
701	SAMNESSKTDQYLCD	ubiquitination	[4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs. 

Sequence Annotation

 NP_BIND 43 48 FAD (By similarity).  

Keyword

 Alternative splicing; Cardiomyopathy; Complete proteome; Disease mutation; FAD; Flavoprotein; Mitochondrion; Reference proteome; Transit peptide; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 717 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0070899; P:mitochondrial tRNA wobble uridine modification; IEA:Compara.
 GO:0055114; P:oxidation-reduction process; IEA:InterPro. 

Interpro

 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR026904; GidA-assoc_3.
 IPR002218; GIDA-rel.
 IPR020595; GIDA-rel_CS. 

Pfam

 PF01134; GIDA
 PF13932; GIDA_assoc_3 

SMART

  

PROSITE

 PS01280; GIDA_1
 PS01281; GIDA_2 

PRINTS

 PR00368; FADPNR.