CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012262
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 7 
Protein Synonyms/Alias
 Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85 
Gene Name
 ARHGEF7 
Gene Synonyms/Alias
 COOL1; KIAA0142; P85SPR; PAK3BP; PIXB; Nbla10314 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
312VLTTGLSKPFMRLDKubiquitination[1]
325DKYPTLLKELERHMEubiquitination[1]
349QKSMAAFKNLSAQCQubiquitination[1]
609DEEFASRKSTAALEEubiquitination[1]
632EAYCTSAKTRQTLNSubiquitination[1, 2]
643TLNSSSRKESAPQVLubiquitination[1]
656VLLPEEEKIIVEETKubiquitination[1]
663KIIVEETKSNGQTVIubiquitination[1, 2]
683VDTVYALKDEVQELRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons. 
Sequence Annotation
 DOMAIN 1 132 CH.
 DOMAIN 184 243 SH3.
 DOMAIN 271 451 DH.
 DOMAIN 473 578 PH.
 MOD_RES 153 153 Phosphoserine (By similarity).
 MOD_RES 176 176 Phosphoserine (By similarity).
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 518 518 Phosphoserine.
 MOD_RES 694 694 Phosphoserine; by CaMK1.  
Keyword
 3D-structure; Alternative splicing; Cell junction; Cell projection; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 803 AA 
Protein Sequence
MNSAEQTVTW LITLGVLESP KKTISDPEGF LQASLKDGVV LCRLLERLLP GTIEKVYPEP 60
RSESECLSNI REFLRGCGAS LRLEDMTDNS NNQLVVRAKF NFQQTNEDEL SFSKGDVIHV 120
TRVEEGGWWE GTLNGRTGWF PSNYVREVKA SEKPVSPKSG TLKSPPKGFD TTAINKSYYN 180
VVLQNILETE NEYSKELQTV LSTYLRPLQT SEKLSSANIS YLMGNLEEIC SFQQMLVQSL 240
EECTKLPEAQ QRVGGCFLNL MPQMKTLYLT YCANHPSAVN VLTEHSEELG EFMETKGASS 300
PGILVLTTGL SKPFMRLDKY PTLLKELERH MEDYHTDRQD IQKSMAAFKN LSAQCQEVRK 360
RKELELQILT EAIRNWEGDD IKTLGNVTYM SQVLIQCAGS EEKNERYLLL FPNVLLMLSA 420
SPRMSGFIYQ GKLPTTGMTI TKLEDSENHR NAFEISGSMI ERILVSCNNQ QDLQEWVEHL 480
QKQTKVTSVG NPTIKPHSVP SHTLPSHPVT PSSKHADSKP APLTPAYHTL PHPSHHGTPH 540
TTINWGPLEP PKTPKPWSLS CLRPAPPLRP SAALCYKEDL SKSPKTMKKL LPKRKPERKP 600
SDEEFASRKS TAALEEDAQI LKVIEAYCTS AKTRQTLNSS SRKESAPQVL LPEEEKIIVE 660
ETKSNGQTVI EEKSLVDTVY ALKDEVQELR QDNKKMKKSL EEEQRARKDL EKLVRKVLKN 720
MNDPAWDETN L 731 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0043005; C:neuron projection; IDA:BHF-UCL.
 GO:0043025; C:neuronal cell body; IDA:BHF-UCL.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:BHF-UCL.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0030032; P:lamellipodium assembly; ISS:UniProtKB.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
 GO:0043547; P:positive regulation of GTPase activity; IDA:BHF-UCL.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR001715; CH-domain.
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00307; CH
 PF00169; PH
 PF00621; RhoGEF
 PF07653; SH3_2 
SMART
 SM00033; CH
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS50021; CH
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.