CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000328
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phospholipase D2 
Protein Synonyms/Alias
 PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2 
Gene Name
 PLD2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80ERYTSGSKVGTCTLYubiquitination[1, 2]
118HRDLLRHKVLMSLLPubiquitination[1]
388WRLDIMLKRKAEEGVubiquitination[3]
517DYSNLITKDWVQLDRubiquitination[3]
656DGRTVLNKVGDEIVDubiquitination[1, 3, 4]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 May have a role in signal-induced cytoskeletal regulation and/or endocytosis (By similarity). 
Sequence Annotation
 DOMAIN 65 195 PX.
 DOMAIN 203 311 PH.
 DOMAIN 437 464 PLD phosphodiesterase 1.
 DOMAIN 751 778 PLD phosphodiesterase 2.
 REGION 441 788 Catalytic.  
Keyword
 Alternative splicing; Complete proteome; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 933 AA 
Protein Sequence
MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ SLKVHPLVFA 60
PGVPVTAQVV GTERYTSGSK VGTCTLYSVR LTHGDFSWTT KKKYRHFQEL HRDLLRHKVL 120
MSLLPLARFA VAYSPARDAG NREMPSLPRA GPEGSTRHAA SKQKYLENYL NRLLTMSFYR 180
NYHAMTEFLE VSQLSFIPDL GRKGLEGMIR KRSGGHRVPG LTCCGRDQVC YRWSKRWLVV 240
KDSFLLYMCL ETGAISFVQL FDPGFEVQVG KRSTEARHGV RIDTSHRSLI LKCSSYRQAR 300
WWAQEITELA QGPGRDFLQL HRHDSYAPPR PGTLARWFVN GAGYFAAVAD AILRAQEEIF 360
ITDWWLSPEV YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL LFKEVELALG INSGYSKRAL 420
MLLHPNIKVM RHPDQVTLWA HHEKLLVVDQ VVAFLGGLDL AYGRWDDLHY RLTDLGDSSE 480
SAASQPPTPR PDSPATPDLS HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW 540
RDVGVVVHGL PARDLARHFI QRWNFTKTTK AKYKTPTYPY LLPKSTSTAN QLPFTLPGGQ 600
CTTVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG RTVLNKVGDE 660
IVDRILKAHK QGWCYRVYVL LPLLPGFEGD ISTGGGNSIQ AILHFTYRTL CRGEYSILHR 720
LKAAMGTAWR DYISICGLRT HGELGGHPVS ELIYIHSKVL IADDRTVIIG SANINDRSLL 780
GKRDSELAVL IEDTETEPSL MNGAEYQAGR FALSLRKHCF GVILGANTRP DLDLRDPICD 840
DFFQLWQDMA ESNANIYEQI FRCLPSNATR SLRTLREYVA VEPLATVSPP LARSELTQVQ 900
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT 933 
Gene Ontology
 GO:0031526; C:brush border membrane; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0070290; F:NAPE-specific phospholipase D activity; IEA:EC.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0004630; F:phospholipase D activity; TAS:ProtInc.
 GO:0007010; P:cytoskeleton organization; TAS:ProtInc.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0002031; P:G-protein coupled receptor internalization; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0006654; P:phosphatidic acid biosynthetic process; TAS:Reactome.
 GO:0006655; P:phosphatidylglycerol biosynthetic process; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR011993; PH_like_dom.
 IPR001683; Phox.
 IPR025202; PLD-like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001736; PLipase_D/transphosphatidylase.
 IPR016555; PLipase_D_euk.
 IPR015679; PLipase_D_fam. 
Pfam
 PF00614; PLDc
 PF13091; PLDc_2
 PF00787; PX 
SMART
 SM00233; PH
 SM00155; PLDc
 SM00312; PX 
PROSITE
 PS50003; PH_DOMAIN
 PS50035; PLD
 PS50195; PX 
PRINTS