CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008854
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Wee1-like protein kinase 
Protein Synonyms/Alias
 Dwee1 
Gene Name
 wee 
Gene Synonyms/Alias
 CG4488 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
594TPFSSHGKFDSISDRacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Could act as a negative regulator of entry into mitosis (G2 to M transition). This kinase specifically phosphorylates and inactivates cyclin B1-complexed CDC2. 
Sequence Annotation
 DOMAIN 239 517 Protein kinase.
 NP_BIND 245 253 ATP (By similarity).
 ACT_SITE 361 361 Proton acceptor (By similarity).
 METAL 366 366 Magnesium; via carbonyl oxygen (By
 METAL 412 412 Magnesium; via carbonyl oxygen (By
 BINDING 268 268 ATP (By similarity).
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 25 25 Phosphoserine.
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 47 47 Phosphothreonine.
 MOD_RES 52 52 Phosphoserine.
 MOD_RES 165 165 Phosphothreonine.
 MOD_RES 168 168 Phosphoserine.  
Keyword
 ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 609 AA 
Protein Sequence
MAFRQSEHEM SVTSLDSSVE LRSRSPSPQV FNPRKLRFAD DDFDKDTPEG ASPQHPLQQR 60
PKLSSGEEQQ LDSKIGKEGG DGDVSMSPPC QKVRALRLFS TPATPKTILQ KSTTQCSNHL 120
SAAAAAVNAS RRSDDLFRLS ERPRSLPLHN RKLPTQDTAN VNPFTPDSLM AHNKKRCRTQ 180
FGRENLNLNV NAMQKYLLSD ACDDDVTEEA GDSMREIHQQ APKRLALHDT NISRFKREFM 240
QVNVIGVGEF GVVFQCVNRL DGCIYAIKKS KKPVAGSSFE KRALNEVWAH AVLGKHDNVV 300
RYYSAWAEDD HMLIQNEFCD GGSLHARIQD HCLGEAELKI VLMHVIEGLR YIHSNDLVHM 360
DLKPENIFST MNPNAHKLVE VQPQQTKDDD GMDSVYEELR HSENLVTYKI GDLGHVTSVK 420
EPYVEEGDCR YLPKEILHED YSNLFKADIF SLGITLFEAA GGGPLPKNGP EWHNLRDGKV 480
PILPSLSRDF NELIAQMMHP YPDKRPTSQS IFSHPILSAV DSKSKLQLGL ELTVEKRKNE 540
ILMNKLREAK KQIKLLEQRV NLLAVTNNPD SLDGQRCLRS FTRRMRTPFS SHGKFDSISD 600
RNKNVITNI 609 
Gene Ontology
 GO:0005875; C:microtubule associated complex; IDA:FlyBase.
 GO:0005634; C:nucleus; IDA:FlyBase.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.
 GO:0004713; F:protein tyrosine kinase activity; IDA:FlyBase.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051642; P:centrosome localization; IMP:FlyBase.
 GO:0051299; P:centrosome separation; IMP:FlyBase.
 GO:0001700; P:embryonic development via the syncytial blastoderm; TAS:FlyBase.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007093; P:mitotic cell cycle checkpoint; IDA:FlyBase.
 GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:FlyBase.
 GO:0051225; P:spindle assembly; IMP:FlyBase. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR017164; Wee1-like_protein_kinase. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS