UniProt Accession

 ENO2_YEAST; P00925; D3DLC2; P99013 

Genbank Protein ID

 J01323; U00027; BK006934 

Genbank Nucleotide ID

 AAA88713.1; AAB68019.1; DAA06866.1 

Protein Name

 Enolase 2 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase 2; 2-phosphoglycerate dehydratase 2 

Gene Name

 ENO2 

Gene Synonyms/Alias

 ENOB; YHR174W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
54	LEMRDEDKSKWMGKG	acetylation	[1]
60	DKSKWMGKGVMNAVN	ubiquitination	[2, 3]
79	VIAAAFVKANLDVKD	ubiquitination	[2, 3]
85	VKANLDVKDQKAVDD	acetylation	[1]
88	NLDVKDQKAVDDFLL	acetylation	[1]
103	SLDGTANKSKLGANA	acetylation	[1]
105	DGTANKSKLGANAIL	acetylation	[1]
126	ARAAAAEKNVPLYQH	acetylation	[1]
139	QHLADLSKSKTSPYV	acetylation	[1]
243	AGHDGKVKIGLDCAS	acetylation	[1]
243	AGHDGKVKIGLDCAS	ubiquitination	[2, 3]
258	SEFFKDGKYDLDFKN	acetylation	[1]
264	GKYDLDFKNPESDKS	acetylation	[1]
270	FKNPESDKSKWLTGV	acetylation	[1]
272	NPESDKSKWLTGVEL	acetylation	[1]
288	DMYHSLMKRYPIVSI	acetylation	[1]
288	DMYHSLMKRYPIVSI	ubiquitination	[3]
358	GTLSESIKAAQDSFA	ubiquitination	[2, 3]
423	IEEELGDKAVYAGEN	acetylation	[1]
423	IEEELGDKAVYAGEN	ubiquitination	[3]
436	ENFHHGDKL******	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

  

Sequence Annotation

 ACT_SITE 160 160 By similarity.
 METAL 247 247 Magnesium (By similarity).
 METAL 296 296 Magnesium (By similarity).
 METAL 321 321 Magnesium (By similarity).
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 104 104 Phosphoserine (By similarity).
 MOD_RES 138 138 Phosphoserine.
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 313 313 Phosphothreonine.
 MOD_RES 324 324 Phosphothreonine.  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 437 AA 

Protein Sequence

Gene Ontology

 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0009898; C:internal side of plasma membrane; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0000015; C:phosphopyruvate hydratase complex; IDA:SGD.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IMP:SGD.
 GO:0006094; P:gluconeogenesis; IEP:SGD.
 GO:0006096; P:glycolysis; IMP:SGD.
 GO:0032889; P:regulation of vacuole fusion, non-autophagic; IDA:SGD. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.