CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010840
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-hydroxy-3-methylglutaryl-coenzyme A reductase 
Protein Synonyms/Alias
 HMG-CoA reductase 
Gene Name
 Hmgcr 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
381LLVRRNQKLSSVEEDubiquitination[1]
459ENAEKGAKFLSDAEIubiquitination[1]
473IIQLVNAKHIPAYKLubiquitination[1]
479AKHIPAYKLETLMETubiquitination[1]
501RRQLLSTKLAEPSSLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins. 
Sequence Annotation
 DOMAIN 61 218 SSD.
 REGION 340 449 Linker.
 REGION 450 887 Catalytic.
 MOTIF 75 78 INSIG-binding motif (By similarity).
 ACT_SITE 558 558 Charge relay system (By similarity).
 ACT_SITE 690 690 Charge relay system (By similarity).
 ACT_SITE 766 766 Charge relay system (By similarity).
 ACT_SITE 865 865 Proton donor (By similarity).
 MOD_RES 871 871 Phosphoserine; by AMPK (By similarity).
 CARBOHYD 281 281 N-linked (GlcNAc...) (Potential).
 CARBOHYD 517 517 N-linked (GlcNAc...) (Potential).
 CARBOHYD 869 869 N-linked (GlcNAc...) (Potential).
 CROSSLNK 89 89 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 248 248 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Endoplasmic reticulum; Glycoprotein; Isopeptide bond; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 887 AA 
Protein Sequence
MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC PKFEEDVLSS 60
DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI FSSFVFSTVV IHFLDKELTG 120
LNEALPFFLL LIDLSRASAL AKFALSSNSQ DEVRENIARG MAILGPTFTL DALVECLVIG 180
VGTMSGVRQL EIMCCFGCMS VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR 240
VLEEEENKPN PVTQRVKMIM SLGLVLVHAH SRWIADPSPQ NSTAEQAKVS LGLDEDVSKR 300
IEPSVSLWQF YLSKMISMDI EQVITLSLAF LLAVKYIFFE QAETESTLSL KNPITSPVVT 360
SKKAQDNCCR REPLLVRRNQ KLSSVEEDPG ANQERKVEVI KPLVVEAETT SRATFVLGAS 420
VASPPSALGT QEPGIELPIE PRPNEECLQI LENAEKGAKF LSDAEIIQLV NAKHIPAYKL 480
ETLMETHERG VSIRRQLLST KLAEPSSLQY LPYRDYNYSL VMGACCENVI GYMPIPVGVA 540
GPLCLDGKEY QVPMATTEGC LVASTNRGCR AISLGGGASS RVLADGMTRG PVVRLPRACD 600
SAEVKTWLET PEGFAVIKEA FDSTSRFARL QKLHVTMAGR NLYIRFQSRT GDAMGMNMIS 660
KGTEKALLKL QEFFPDMQIL AVSGNYCTDK KPAAINWIEG RGKTVVCEAV IPAKVVREVL 720
KTTTEAMVDV NINKNLVGSA MAGSIGGYNA HAANIVTAIY IACGQDAAQN VGSSNCITLM 780
EASGPTNEDL YISCTMPSIE IGTVGGGTNL LPQQACLQML GVQGACKDNP GENARQLARI 840
VCGTVMAGEL SLMAALAAGH LVRSHMVHNR SKINLQDLQG TCTKKAA 887 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IDA:MGI.
 GO:0042282; F:hydroxymethylglutaryl-CoA reductase activity; IEA:Compara.
 GO:0070402; F:NADPH binding; IEA:Compara.
 GO:0007568; P:aging; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; IDA:MGI.
 GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
 GO:0009790; P:embryo development; IMP:MGI.
 GO:0008299; P:isoprenoid biosynthetic process; IEA:Compara.
 GO:0045445; P:myoblast differentiation; IEA:Compara.
 GO:0061179; P:negative regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Compara.
 GO:0043407; P:negative regulation of MAP kinase activity; IGI:MGI.
 GO:0010664; P:negative regulation of striated muscle cell apoptotic process; IEA:Compara.
 GO:0045908; P:negative regulation of vasodilation; IEA:Compara.
 GO:0061045; P:negative regulation of wound healing; IEA:Compara.
 GO:0010666; P:positive regulation of cardiac muscle cell apoptotic process; IEA:Compara.
 GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IEA:Compara.
 GO:0048643; P:positive regulation of skeletal muscle tissue development; IEA:Compara.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0032874; P:positive regulation of stress-activated MAPK cascade; IEA:Compara.
 GO:0051262; P:protein tetramerization; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0006743; P:ubiquinone metabolic process; IEA:Compara.
 GO:0008542; P:visual learning; IGI:MGI. 
Interpro
 IPR002202; HMG_CoA_Rdtase.
 IPR023074; HMG_CoA_Rdtase_cat.
 IPR023076; HMG_CoA_Rdtase_CS.
 IPR004554; HMG_CoA_Rdtase_eu_arc.
 IPR004816; HMG_CoA_Rdtase_metazoan.
 IPR023282; HMG_CoA_Rdtase_N.
 IPR009023; HMG_CoA_Rdtase_NAD(P)-bd.
 IPR009029; HMG_CoA_Rdtase_sub-bd.
 IPR000731; SSD. 
Pfam
 PF00368; HMG-CoA_red 
SMART
  
PROSITE
 PS00066; HMG_COA_REDUCTASE_1
 PS00318; HMG_COA_REDUCTASE_2
 PS01192; HMG_COA_REDUCTASE_3
 PS50065; HMG_COA_REDUCTASE_4
 PS50156; SSD 
PRINTS
 PR00071; HMGCOARDTASE.