UniProt Accession

 ECI1_MOUSE; P42125; Q8QZV3 

Genbank Protein ID

 Z14049; Z14050; Z14051; Z14052; Z14053; Z14054; AK029481; AC154237; CH466606; BC022712; BC054444 

Genbank Nucleotide ID

 CAA78417.1; CAA78418.1; CAA78418.1; CAA78418.1; CAA78418.1; CAA78418.1; BAC26469.1; EDL22317.1; AAH22712.1; AAH54444.1 

Protein Name

 Enoyl-CoA delta isomerase 1, mitochondrial 

Protein Synonyms/Alias

 3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase 

Gene Name

 Eci1 

Gene Synonyms/Alias

 Dci 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
48	PAGVAVMKLRNPPVN	acetylation	[1, 2, 3]
48	PAGVAVMKLRNPPVN	succinylation	[2]
71	EFTISLEKLENDKSI	acetylation	[2, 4]
71	EFTISLEKLENDKSI	succinylation	[2]
76	LEKLENDKSIRGVIL	acetylation	[1, 2, 3, 4, 5, 6]
76	LEKLENDKSIRGVIL	succinylation	[2]
113	AHYAEYWKNVQELWL	acetylation	[1, 3]
158	RVMADNPKYTIGLNE	acetylation	[2]
158	RVMADNPKYTIGLNE	succinylation	[2]
206	FSPAEALKVGVVDEV	acetylation	[1]
222	PEDQVHSKARSVMTK	acetylation	[1, 2, 3, 4, 6, 7]
222	PEDQVHSKARSVMTK	succinylation	[2]
229	KARSVMTKWLAIPDH	acetylation	[1, 2, 3, 4, 5, 6, 7, 8, 9]
229	KARSVMTKWLAIPDH	succinylation	[2]
255	ATADNLIKQREADIQ	acetylation	[1, 2, 3, 6]
255	ATADNLIKQREADIQ	succinylation	[2]
270	NFTSFISKDSIQKSL	acetylation	[1, 2, 3, 4, 5, 6, 9, 10]
270	NFTSFISKDSIQKSL	succinylation	[2]
270	NFTSFISKDSIQKSL	ubiquitination	[11]
275	ISKDSIQKSLHMYLE	acetylation	[1, 2, 3, 6]
275	ISKDSIQKSLHMYLE	succinylation	[2]
283	SLHMYLEKLKQKKG*	acetylation	[1, 2, 3, 6]
283	SLHMYLEKLKQKKG*	succinylation	[2]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [6] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [8] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [9] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [11] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. 

Sequence Annotation

 REGION 93 97 Substrate binding (By similarity).
 BINDING 140 140 Substrate; via amide nitrogen (By
 BINDING 164 164 Substrate (By similarity).
 MOD_RES 76 76 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Fatty acid metabolism; Isomerase; Lipid metabolism; Mitochondrion; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 289 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IDA:MGI.
 GO:0006635; P:fatty acid beta-oxidation; IMP:MGI. 

Interpro

 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 

Pfam

 PF00378; ECH 

SMART

  

PROSITE

 PS00166; ENOYL_COA_HYDRATASE 

PRINTS