UniProt Accession

 J3QN31_MOUSE; J3QN31 

Genbank Protein ID

 AC124373 

Genbank Nucleotide ID

  

Protein Name

 Adenylosuccinate synthetase isozyme 1 

Protein Synonyms/Alias

 AMPSase 1; AdSS 1; Adenylosuccinate synthetase, basic isozyme; Adenylosuccinate synthetase, muscle isozyme; IMP--aspartate ligase 1 

Gene Name

 Adssl1 

Gene Synonyms/Alias

 Adss1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
18	PPGTGGVKRGRLQQE	ubiquitination	[1]
48	WGDEGKGKVVDLLAT	ubiquitination	[1]
181	QRQAQEGKNIGTTKK	ubiquitination	[1]
197	IGPTYSSKAARTGLR	ubiquitination	[1]
433	YETLPGWKADTTGAR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP (By similarity). 

Sequence Annotation

 NP_BIND 42 48 GTP (By similarity).
 NP_BIND 70 72 GTP (By similarity).
 NP_BIND 386 388 GTP (By similarity).
 NP_BIND 468 471 GTP (By similarity).
 REGION 43 46 IMP binding (By similarity).
 REGION 68 71 IMP binding (By similarity).
 REGION 354 360 Substrate binding (By similarity).
 ACT_SITE 43 43 Proton acceptor (By similarity).
 ACT_SITE 71 71 Proton donor (By similarity).
 METAL 43 43 Magnesium (By similarity).
 METAL 70 70 Magnesium; via carbonyl oxygen (By
 BINDING 43 43 Substrate (By similarity).
 BINDING 186 186 IMP (By similarity).
 BINDING 200 200 IMP; shared with dimeric partner (By
 BINDING 279 279 IMP (By similarity).
 BINDING 294 294 IMP (By similarity).
 BINDING 358 358 IMP (By similarity).
 BINDING 360 360 GTP (By similarity).  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 480 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0004019; F:adenylosuccinate synthase activity; IDA:MGI.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0044208; P:'de novo' AMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006163; P:purine nucleotide metabolic process; IDA:MGI. 

Interpro

 IPR018220; Adenylosuccinate_synthase_AS.
 IPR001114; Adenylosuccinate_synthetase.
 IPR027509; AdSS_1_vert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00709; Adenylsucc_synt 

SMART

 SM00788; Adenylsucc_synt 

PROSITE

 PS01266; ADENYLOSUCCIN_SYN_1
 PS00513; ADENYLOSUCCIN_SYN_2 

PRINTS