CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014713
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor E4F1 
Protein Synonyms/Alias
 E4F transcription factor 1; Putative E3 ubiquitin-protein ligase E4F1; Transcription factor E4F; p120E4F; p50E4F 
Gene Name
 E4F1 
Gene Synonyms/Alias
 E4F 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
188QVKLLVNKDGRYVCAubiquitination[1]
289SKDVVVSKEDARAGSubiquitination[1]
501GDCGKLYKTIAHVRGubiquitination[1]
545FRTHLEEKPHVCQFCubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription. 
Sequence Annotation
 ZN_FING 192 214 C2H2-type 1.
 ZN_FING 220 242 C2H2-type 2.
 ZN_FING 248 272 C2H2-type 3; degenerate.
 ZN_FING 435 457 C2H2-type 4.
 ZN_FING 463 485 C2H2-type 5.
 ZN_FING 491 513 C2H2-type 6.
 ZN_FING 519 541 C2H2-type 7.
 ZN_FING 547 569 C2H2-type 8.
 ZN_FING 575 597 C2H2-type 9; degenerate.
 REGION 41 85 Required for ubiquitin ligase activity.
 REGION 184 263 Mediates dimerization, DNA-binding,
 REGION 369 566 Mediates interaction with CDKN2A.
 REGION 435 599 Interaction with BMI1.
 REGION 521 580 Mediates interaction with TP53.
 REGION 575 597 Mediates interaction with RASSF1.
 MOD_RES 50 50 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; DNA-binding; Growth regulation; Host-virus interaction; Ligase; Metal-binding; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 784 AA 
Protein Sequence
MEGAMAVRVT AAHTAEAQAE AGREAGEGAV AAVAAALAPS GFLGLPAPFS EEDEDDVHRC 60
GRCQAEFTAL EDFVQHKIQK ACQRAPPEAL PATPATTALL GQEVVPAAPG PEEPITVAHI 120
VVEAASLAAD ISHASDLVGG GHIKEVIVAA EAELGDGEMA EAPGSPRQQG LGLAGEGEQA 180
QVKLLVNKDG RYVCALCHKT FKTGSILKAH MVTHSSRKDH ECKLCGASFR TKGSLIRHHR 240
RHTDERPYKC SKCGKSFRES GALTRHLKSL TPCTEKIRFS VSKDVVVSKE DARAGSGAGA 300
AGLGTATSSV TGEPIETSPV IHLVTDAKGT VIHEVHVQMQ ELSLGMKALA PEPPVSQELP 360
CSSEGSRENL LHQAMQNSGI VLERAAGEEG ALEPAPAAGS SPQPLAVAAP QLPVLEVQPL 420
ETQVASEASA VPRTHPCPQC SETFPTAATL EAHKRGHTGP RPFACAQCGK AFPKAYLLKK 480
HQEVHVRERR FRCGDCGKLY KTIAHVRGHR RVHSDERPYP CPKCGKRYKT KNAQQVHFRT 540
HLEEKPHVCQ FCSRGFREKG SLVRHVRHHT GEKPFKCYKC GRGFAEHGTL NRHLRTKGGC 600
LLEVEELLVS EDSPAAATTV LTEDPHTVLV EFSSVVADTQ EYIIEATADD AETSEATEII 660
EGTQTEVDSH IMKVVQQIVH QASAGHQIIV QNVTMDEETA LGPEAAAADT ITIATPESLT 720
EQVAMTLASA ISEGTVLAAR AGTSGTEQAT VTMVSSEDIE ILEHAGELVI ASPEGQLEVQ 780
TVIV 784 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IEA:Compara.
 GO:0035497; F:cAMP response element binding; IDA:UniProtKB.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0006260; P:DNA replication; IEA:Compara.
 GO:0009790; P:embryo development; IEA:Compara.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0071850; P:mitotic cell cycle arrest; TAS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0010564; P:regulation of cell cycle process; IDA:UniProtKB.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
 GO:0009794; P:regulation of mitotic cell cycle, embryonic; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS