CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013943
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polynucleotide 5'-hydroxyl-kinase NOL9 
Protein Synonyms/Alias
 Nucleolar protein 9 
Gene Name
 NOL9 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22STWLRVRKARPQLILubiquitination[1, 2]
376QKMVYYGKPSCKNNYubiquitination[1]
440QFRSDHSKYMPDLTPubiquitination[1, 3]
459DMDGLYTKSKTKMRNubiquitination[3]
461DGLYTKSKTKMRNRRubiquitination[1]
485LEFADEEKESPVEFTubiquitination[1]
618CRGIDMEKRLYHILTubiquitination[1]
651AIPHCVLKCQRGIEGubiquitination[1, 2]
670VTTDYNFKLPGASEKubiquitination[1, 3, 4, 5]
677KLPGASEKIGAREPEubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA. 
Sequence Annotation
 NP_BIND 306 313 ATP (Probable).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 487 487 Phosphoserine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; rRNA processing; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 702 AA 
Protein Sequence
MADSGLLLKR GSCRSTWLRV RKARPQLILS RRPRRRLGSL RWCGRRRLRW RLLQAQASGV 60
DWREGARQVS RAAAARRPNT ATPSPIPSPT PASEPESEPE LESASSCHRP LLIPPVRPVG 120
PGRALLLLPV EQGFTFSGIC RVTCLYGQVQ VFGFTISQGQ PAQDIFSVYT HSCLSIHALH 180
YSQPEKSKKE LKREARNLLK SHLNLDDRRW SMQNFSPQCS IVLLEHLKTA TVNFITSYPG 240
SSYIFVQESP TPQIKPEYLA LRSVGIRREK KRKGLQLTES TLSALEELVN VSCEEVDGCP 300
VILVCGSQDV GKSTFNRYLI NHLLNSLPCV DYLECDLGQT EFTPPGCISL LNITEPVLGP 360
PFTHLRTPQK MVYYGKPSCK NNYENYIDIV KYVFSAYKRE SPLIVNTMGW VSDQGLLLLI 420
DLIRLLSPSH VVQFRSDHSK YMPDLTPQYV DDMDGLYTKS KTKMRNRRFR LAAFADALEF 480
ADEEKESPVE FTGHKLIGVY TDFAFRITPR NRESHNKILR DLSILSYLSQ LQPPMPKPLS 540
PLHSLTPYQV PFNAVALRIT HSDVAPTHIL YAVNASWVGL CKIQDDVRGY TNGPILLAQT 600
PICDCLGFGI CRGIDMEKRL YHILTPVPPE ELRTVNCLLV GAIAIPHCVL KCQRGIEGTV 660
PYVTTDYNFK LPGASEKIGA REPEEAHKEK PYRRPKFCRK MK 702 
Gene Ontology
 GO:0005730; C:nucleolus; TAS:UniProtKB.
 GO:0005524; F:ATP binding; TAS:UniProtKB.
 GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000460; P:maturation of 5.8S rRNA; IMP:UniProtKB. 
Interpro
 IPR010655; Pre-mRNA_cleavage_cplxII_Clp1. 
Pfam
 PF06807; Clp1 
SMART
  
PROSITE
  
PRINTS