CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024597
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit I 
Protein Synonyms/Alias
 eIF3i; Eukaryotic translation initiation factor 3 subunit 2; eIF-3-beta; eIF3 p36 
Gene Name
 Eif3i 
Gene Synonyms/Alias
 Eif3s2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
2******MKPILLQGHubiquitination[1]
85LWDCETGKQLALLKTubiquitination[1]
282EEEFGRVKGHFGPINacetylation[2]
282EEEFGRVKGHFGPINubiquitination[1]
298VAFHPDGKSYSSGGEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity). 
Sequence Annotation
 REPEAT 8 47 WD 1.
 REPEAT 50 91 WD 2.
 REPEAT 144 183 WD 3.
 REPEAT 186 225 WD 4.
 REPEAT 283 324 WD 5.
 MOD_RES 264 264 N6-acetyllysine (By similarity).
 MOD_RES 308 308 Phosphotyrosine (By similarity).
 CROSSLNK 282 282 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Initiation factor; Isopeptide bond; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; Ubl conjugation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 325 AA 
Protein Sequence
MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDA 60
DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG FDFGGNIIMF STDKQMGYQC 120
FVSFFDLRDP SQIDSNEPYM KIPCNDSKIT SAVWGPLGEC IIAGHESGEL NQYSAKSGEV 180
LVNVKEHSRQ INDIQLSRDM TMFVTASKDN TAKLFDSTSL EHQKTFRTER PVNSAALSPN 240
YDHVVLGGGQ EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY 300
SSGGEDGYVR IHYFDPQYFE FEFEA 325 
Gene Ontology
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB.
 GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 
Interpro
 IPR027525; eIF3i.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS