CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017863
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Endoribonuclease Dicer 
Protein Synonyms/Alias
 Double-strand-specific ribonuclease mDCR-1 
Gene Name
 Dicer1 
Gene Synonyms/Alias
 Dicer; Mdcr 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
652PFTHLAPKCRTRELPubiquitination[1]
1462GSGAFVRKISLSPFSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs (By similarity). 
Sequence Annotation
 DOMAIN 51 227 Helicase ATP-binding.
 DOMAIN 433 602 Helicase C-terminal.
 DOMAIN 630 722 Dicer dsRNA-binding fold.
 DOMAIN 891 1042 PAZ.
 DOMAIN 1276 1403 RNase III 1.
 DOMAIN 1660 1818 RNase III 2.
 DOMAIN 1843 1908 DRBM.
 NP_BIND 64 71 ATP (By similarity).
 REGION 256 595 Required for interaction with PRKRA and
 MOTIF 175 178 DECH box.
 METAL 1316 1316 Magnesium or manganese 1 (By similarity).
 METAL 1395 1395 Magnesium or manganese 1 (By similarity).
 METAL 1398 1398 Magnesium or manganese 1 (By similarity).
 METAL 1699 1699 Magnesium or manganese 2.
 METAL 1804 1804 Magnesium or manganese 2.
 METAL 1807 1807 Magnesium or manganese 2.
 MOD_RES 413 413 Phosphoserine (By similarity).
 MOD_RES 415 415 Phosphoserine (By similarity).
 MOD_RES 1016 1016 Phosphoserine (By similarity).  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Endonuclease; Helicase; Hydrolase; Magnesium; Manganese; Metal-binding; Nuclease; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; RNA-binding; RNA-mediated gene silencing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1916 AA 
Protein Sequence
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT 60
IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PHAKRTVFLV NSANQVAQQV SAVRTHSDLK 120
VGEYSDLEVN ASWTKERWSQ EFTKHQVLIM TCYVALTVLK NGYLSLSDIN LLVFDECHLA 180
ILDHPYREIM KLCESCPSCP RILGLTASIL NGKCDPEELE EKIQKLERIL RSDAETATDL 240
VVLDRYTSQP CEIVVDCGPF TDRSGLYERL LMELEAALDF INDCNVAVHS KERDSTLISK 300
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT LLRKIHALCE 360
EYFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW YNNRNQDNYV SWSDSEDDDD 420
DEEIEEKEKP ETNFPSPFTN ILCGIIFVER RYTAVVLNRL IKEAGKQDPE LAYISSNFIT 480
GHGIGKNQPR SKQMEAEFRK QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP 540
TEYRSYVQSK GRARAPISNY VMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSADGAEAD 600
VHAGVDDEDA FPPYVLRPDD GGPRVTINTA IGHINRYCAR LPSDPFTHLA PKCRTRELPD 660
GTFYSTLYLP INSPLRASIV GPPMDSVRLA ERVVALICCE KLHKIGELDE HLMPVGKETV 720
KYEEELDLHD EEETSVPGRP GSTKRRQCYP KAIPECLRES YPKPDQPCYL YVIGMVLTTP 780
LPDELNFRRR KLYPPEDTTR CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ 840
QMLELITRLH QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSGTL DIDFKFMEDI 900
EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV YTDLTPLSKF 960
PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL TPRHLNQKGK ALPLSSAEKR 1020
KAKWESLQNK QILVPELCAI HPIPASLWRK AVCLPSILYR LHCLLTAEEL RAQTASDAGV 1080
GVRSLPVDFR YPNLDFGWKK SIDSKSFIST CNSSLAESDN YCKHSTTVVP EHAAHQGATR 1140
PSLENHDQMS VNCKRLPAES PAKLQSEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN 1200
QLNYFKQEIP VQPTTSYPIQ NLYNYENQPK PSNECPLLSN TYLDGNANTS TSDGSPAVST 1260
MPAMMNAVKA LKDRMDSEQS PSVGYSSRTL GPNPGLILQA LTLSNASDGF NLERLEMLGD 1320
SFLKHAITTY LFCTYPDAHE GRLSYMRSKK VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW 1380
LPPGYVVNQD KSNSEKWEKD EMTKDCLLAN GKLGEACEEE EDLTWRAPKE EAEDEDDFLE 1440
YDQEHIQFID SMLMGSGAFV RKISLSPFSA SDSAYEWKMP KKASLGSMPF ASGLEDFDYS 1500
SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI ADKSIADCVE 1560
ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALD PAQENGSSQQ KSLSGSCAAP 1620
VGPRSSAGKD LEYGCLKIPP RCMFDHPDAE KTLNHLISGF ETFEKKINYR FKNKAYLLQA 1680
FTHASYHYNT ITDCYQRLEF LGDAILDYLI TKHLYEDPRQ HSPGVLTDLR SALVNNTIFA 1740
SLAVKYDYHK YFKAVSPELF HVIDDFVKFQ LEKNEMQGMD SELRRSEEDE EKEEDIEVPK 1800
AMGDIFESLA GAIYMDSGMS LEVVWQVYYP MMQPLIEKFS ANVPRSPVRE LLEMEPETAK 1860
FSPAERTYDG KVRVTVEVVG KGKFKGVGRS YRIAKSAAAR RALRSLKANQ PQVPNS 1916 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016442; C:RNA-induced silencing complex; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035198; F:miRNA binding; IDA:MGI.
 GO:0004525; F:ribonuclease III activity; ISS:UniProtKB.
 GO:0001525; P:angiogenesis; IMP:MGI.
 GO:0006915; P:apoptotic process; IMP:MGI.
 GO:0048754; P:branching morphogenesis of an epithelial tube; IMP:MGI.
 GO:0055013; P:cardiac muscle cell development; IMP:MGI.
 GO:0021987; P:cerebral cortex development; IMP:MGI.
 GO:0051607; P:defense response to virus; IMP:MGI.
 GO:0035116; P:embryonic hindlimb morphogenesis; IMP:MGI.
 GO:0071335; P:hair follicle cell proliferation; IMP:MGI.
 GO:0031069; P:hair follicle morphogenesis; IMP:MGI.
 GO:0060119; P:inner ear receptor cell development; IMP:MGI.
 GO:0060576; P:intestinal epithelial cell development; IGI:MGI.
 GO:0030324; P:lung development; IMP:MGI.
 GO:0035264; P:multicellular organism growth; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0010626; P:negative regulation of Schwann cell proliferation; IMP:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0021675; P:nerve development; IMP:BHF-UCL.
 GO:0048812; P:neuron projection morphogenesis; IMP:BHF-UCL.
 GO:0021889; P:olfactory bulb interneuron differentiation; IMP:MGI.
 GO:0032290; P:peripheral nervous system myelin formation; IMP:BHF-UCL.
 GO:2000630; P:positive regulation of miRNA metabolic process; IMP:MGI.
 GO:0031643; P:positive regulation of myelination; IMP:BHF-UCL.
 GO:0014040; P:positive regulation of Schwann cell differentiation; IMP:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0009791; P:post-embryonic development; IMP:MGI.
 GO:0031054; P:pre-miRNA processing; ISS:UniProtKB.
 GO:0030422; P:production of siRNA involved in RNA interference; ISS:UniProtKB.
 GO:0051726; P:regulation of cell cycle; IMP:MGI.
 GO:0070173; P:regulation of enamel mineralization; IMP:MGI.
 GO:0045664; P:regulation of neuron differentiation; IMP:MGI.
 GO:0008593; P:regulation of Notch signaling pathway; IC:BHF-UCL.
 GO:0048713; P:regulation of oligodendrocyte differentiation; IMP:MGI.
 GO:0001932; P:regulation of protein phosphorylation; IMP:BHF-UCL.
 GO:0045069; P:regulation of viral genome replication; IMP:MGI.
 GO:0016075; P:rRNA catabolic process; IEA:InterPro.
 GO:0021522; P:spinal cord motor neuron differentiation; IMP:MGI.
 GO:0051225; P:spindle assembly; IMP:MGI.
 GO:0048536; P:spleen development; IMP:MGI.
 GO:0019827; P:stem cell maintenance; IMP:MGI.
 GO:0030423; P:targeting of mRNA for destruction involved in RNA interference; ISS:UniProtKB.
 GO:0010070; P:zygote asymmetric cell division; IMP:MGI. 
Interpro
 IPR005034; Dicer_dsRNA_binding_fold.
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR003100; PAZ.
 IPR011907; RNase_III.
 IPR000999; RNase_III_dom. 
Pfam
 PF00270; DEAD
 PF03368; dsRNA_bind
 PF00271; Helicase_C
 PF02170; PAZ
 PF00636; Ribonuclease_3 
SMART
 SM00487; DEXDc
 SM00358; DSRM
 SM00490; HELICc
 SM00949; PAZ
 SM00535; RIBOc 
PROSITE
 PS51327; DICER_DSRBF
 PS50137; DS_RBD
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS50821; PAZ
 PS00517; RNASE_3_1
 PS50142; RNASE_3_2 
PRINTS