CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024204
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Extended synaptotagmin-2 
Protein Synonyms/Alias
 E-Syt2; Chr2Syt 
Gene Name
 ESYT2 
Gene Synonyms/Alias
 FAM62B; KIAA1228 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
200ERAEWLNKTVKHMWPubiquitination[1]
239LSTFSFTKVDVGQQPubiquitination[2]
253PLRINGVKVYTENVDubiquitination[3]
261VYTENVDKRQIILDLubiquitination[3]
384QLRFPVPKGVLRIHFubiquitination[1]
400EAQDLQGKDTYLKGLubiquitination[1, 3]
405QGKDTYLKGLVKGKSubiquitination[1, 2]
411LKGLVKGKSDPYGIIubiquitination[1, 4]
431IFQSRVIKENLSPKWubiquitination[1]
498LDEVPKGKLHLRLEWubiquitination[1]
553RNLPSGKKISSNPNPubiquitination[3]
578QESKIRYKTNEPVWEubiquitination[1]
647SGPNSTIKMKIALRVubiquitination[1]
649PNSTIKMKIALRVLHubiquitination[1]
659LRVLHLEKRERPPDHubiquitination[1]
875RTLDVAVKNSGGFLSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May play a role as calcium-regulated intrinsic membrane protein. 
Sequence Annotation
 DOMAIN 372 473 C2 1.
 DOMAIN 523 617 C2 2.
 DOMAIN 788 892 C2 3.
 MOD_RES 691 691 Phosphoserine.
 MOD_RES 738 738 Phosphoserine (By similarity).
 MOD_RES 739 739 Phosphoserine.
 MOD_RES 743 743 Phosphoserine.
 MOD_RES 748 748 Phosphoserine.
 MOD_RES 755 755 Phosphoserine.
 MOD_RES 758 758 Phosphoserine.
 MOD_RES 761 761 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 921 AA 
Protein Sequence
MTANRDAALS SHRHPGCAQR PRTPTFASSS QRRSAFGFDD GNFPGLGERS HAPGSRLGAR 60
RRAKTARGLR GHRQRGAGAG LSRPGSARAP SPPRPGGPEN PGGVLSVELP GLLAQLARSF 120
ALLLPVYALG YLGLSFSWVL LALALLAWCR RSRGLKALRL CRALALLEDE ERVVRLGVRA 180
CDLPAWVHFP DTERAEWLNK TVKHMWPFIC QFIEKLFRET IEPAVRGANT HLSTFSFTKV 240
DVGQQPLRIN GVKVYTENVD KRQIILDLQI SFVGNCEIDL EIKRYFCRAG VKSIQIHGTM 300
RVILEPLIGD MPLVGALSIF FLRKPLLEIN WTGLTNLLDV PGLNGLSDTI ILDIISNYLV 360
LPNRITVPLV SEVQIAQLRF PVPKGVLRIH FIEAQDLQGK DTYLKGLVKG KSDPYGIIRV 420
GNQIFQSRVI KENLSPKWNE VYEALVYEHP GQELEIELFD EDPDKDDFLG SLMIDLIEVE 480
KERLLDEWFT LDEVPKGKLH LRLEWLTLMP NASNLDKVLT DIKADKDQAN DGLSSALLIL 540
YLDSARNLPS GKKISSNPNP VVQMSVGHKA QESKIRYKTN EPVWEENFTF FIHNPKRQDL 600
EVEVRDEQHQ CSLGNLKVPL SQLLTSEDMT VSQRFQLSNS GPNSTIKMKI ALRVLHLEKR 660
ERPPDHQHSA QVKRPSVSKE GRKTSIKSHM SGSPGPGGSN TAPSTPVIGG SDKPGMEEKA 720
QPPEAGPQGL HDLGRSSSSL LASPGHISVK EPTPSIASDI SLPIATQELR QRLRQLENGT 780
TLGQSPLGQI QLTIRHSSQR NKLIVVVHAC RNLIAFSEDG SDPYVRMYLL PDKRRSGRRK 840
THVSKKTLNP VFDQSFDFSV SLPEVQRRTL DVAVKNSGGF LSKDKGLLGK VLVALASEEL 900
AKGWTQWYDL TEDGTRPQAM T 921 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting. 
Pfam
 PF00168; C2 
SMART
 SM00239; C2 
PROSITE
 PS50004; C2 
PRINTS