CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017173
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Splicing factor 45 
Protein Synonyms/Alias
 45 kDa-splicing factor; RNA-binding motif protein 17 
Gene Name
 Rbm17 
Gene Synonyms/Alias
 Spf45 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
256REGQGLGKHEQGLSTacetylation[1]
395ACFYNLDKFRVLDLAacetylation[1]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites (By similarity). 
Sequence Annotation
 DOMAIN 235 283 G-patch.
 DOMAIN 310 389 RRM.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 21 21 N6-acetyllysine (By similarity).
 MOD_RES 71 71 Phosphothreonine (By similarity).
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 169 169 Phosphoserine (By similarity).
 MOD_RES 222 222 Phosphoserine (By similarity).  
Keyword
 Acetylation; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 405 AA 
Protein Sequence
MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG 60
GSSDDRQIAD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ 120
RQRELERQKE IEEREKRRKD RHEASGFSRR PDPDSDEDED YERERRKRSM GGAAIAPPTS 180
LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEPDRP RSPTGPSNSF LANMGGTVAH 240
KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKGEAQD ASKKSDSNPL 300
TEILKCPTKV VLLRNMVGAG EVDEDLEVET KEECEKYGKV GKCVIFEIPG APDDEAVRIF 360
LEFERVESAI KAVVDLNGRY FGGRVVKACF YNLDKFRVLD LAEQV 405 
Gene Ontology
 GO:0005681; C:spliceosomal complex; TAS:ProtInc.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; TAS:ProtInc.
 GO:0000375; P:RNA splicing, via transesterification reactions; TAS:UniProtKB. 
Interpro
 IPR000467; G_patch_dom.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR003954; RRM_dom_euk.
 IPR016967; Splicing_factor_SPF45. 
Pfam
 PF01585; G-patch 
SMART
 SM00443; G_patch
 SM00361; RRM_1 
PROSITE
 PS50174; G_PATCH
 PS50102; RRM 
PRINTS