CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016229
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acetoacetyl-CoA synthetase 
Protein Synonyms/Alias
 Acyl-CoA synthetase family member 1; Protein sur-5 homolog 
Gene Name
 AACS 
Gene Synonyms/Alias
 ACSF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
95ADVPEWFKGSRLNYAubiquitination[1, 2, 3]
228EKLQQVVKGLPDLKKubiquitination[2]
530DYCRINPKTGGIVMLubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity). 
Sequence Annotation
 MOD_RES 524 524 Phosphotyrosine (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Fatty acid metabolism; Ligase; Lipid metabolism; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 672 AA 
Protein Sequence
MSKEERPGRE EILECQVMWE PDSKKNTQMD RFRAAVGAAC GLALESYDDL YHWSVESYSD 60
FWAEFWKFSG IVFSRVYDEV VDTSKGIADV PEWFKGSRLN YAENLLRHKE NDRVALYIAR 120
EGKEEIVKVT FEELRQEVAL FAAAMRKMGV KKGDRVVGYL PNSEHAVEAM LAAASIGAIW 180
SSTSPDFGVN GVLDRFSQIQ PKLIFSVEAV VYNGKEHNHM EKLQQVVKGL PDLKKVVVIP 240
YVSSRENIDL SKIPNSVFLD DFLATGTSEQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM 300
VHSAGGTLIQ HLKEHLLHGN MTSSDILLCY TTVGWMMWNW MVSLLATGAA MVLYDGSPLV 360
PTPNVLWDLV DRIGITVLVT GAKWLSVLEE KAMKPVETHS LQMLHTILST GSPLKAQSYE 420
YVYRCIKSSI LLGSISGGTD IISCFMGHNF SLPVYKGEIQ ARNLGMAVEA WNEEGKAVWG 480
ESGELVCTKP IPCQPTHFWN DENGNKYRKA YFSKFPGIWA HGDYCRINPK TGGIVMLGRS 540
DGTLNPNGVR FGSSEIYNIV ESFEEVEDSL CVPQYNKYRE ERVILFLKMA SGHAFQPDLV 600
KRIRDAIRMG LSARHVPSLI LETKGIPYTL NGKKVEVAVK QIIAGKAVEQ GGAFSNPETL 660
DLYRDIPELQ GF 672 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0030729; F:acetoacetate-CoA ligase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047760; F:butyrate-CoA ligase activity; IEA:Compara.
 GO:0060612; P:adipose tissue development; IEA:Compara.
 GO:0071397; P:cellular response to cholesterol; IEA:Compara.
 GO:0071333; P:cellular response to glucose stimulus; IEA:Compara.
 GO:0071394; P:cellular response to testosterone stimulus; IEA:Compara.
 GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0034201; P:response to oleic acid; IEA:Compara.
 GO:0014074; P:response to purine-containing compound; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara.
 GO:0050872; P:white fat cell differentiation; IEA:Compara. 
Interpro
 IPR005914; Acac_CoA_synth.
 IPR024597; Acyl-CoA_synth_DUF3448.
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig. 
Pfam
 PF00501; AMP-binding
 PF11930; DUF3448 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS